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Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups
The objective of this study is to develop an easy and simple diffusion-controlled fabrication technique to generate the concentration gradient of biomolecules in hydrogels. Polyacrylamide (PAAm) hydrogels with a concentration gradient of type I collagen were prepared to evaluate the cell adhesion. T...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445852/ http://dx.doi.org/10.3390/ma3042393 |
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author | Yamamoto, Masaya Yanase, Kaoru Tabata, Yasuhiko |
author_facet | Yamamoto, Masaya Yanase, Kaoru Tabata, Yasuhiko |
author_sort | Yamamoto, Masaya |
collection | PubMed |
description | The objective of this study is to develop an easy and simple diffusion-controlled fabrication technique to generate the concentration gradient of biomolecules in hydrogels. Polyacrylamide (PAAm) hydrogels with a concentration gradient of type I collagen were prepared to evaluate the cell adhesion. The PAAm hydrogel was exposed to a gradient concentration of sodium hydroxide (NaOH) solution at 52 °C to generate that of carboxyl groups in the hydrogel. The carboxyl groups generated were chemically coupled with the amino groups of type I collagen to prepare the hydrogel with a concentration gradient of collagen immobilized. The attachment of L929 fibroblasts was evaluated for the collagen-immobilized hydrogel prepared. The amount gradient of carboxyl groups in the hydrogel increased with an increase in the NaOH concentration while the carboxyl groups gradient enabled to generate a gradient of collagen immobilized in the hydrogel. On the other hand, the number of fibroblasts adhered depended on the amount of collagen immobilized. These findings indicate that the adhesion behavior of cells is modified by the concentration gradient of biomolecule in the three-dimensional scaffold of cells. |
format | Online Article Text |
id | pubmed-5445852 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International |
record_format | MEDLINE/PubMed |
spelling | pubmed-54458522017-07-28 Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups Yamamoto, Masaya Yanase, Kaoru Tabata, Yasuhiko Materials (Basel) Article The objective of this study is to develop an easy and simple diffusion-controlled fabrication technique to generate the concentration gradient of biomolecules in hydrogels. Polyacrylamide (PAAm) hydrogels with a concentration gradient of type I collagen were prepared to evaluate the cell adhesion. The PAAm hydrogel was exposed to a gradient concentration of sodium hydroxide (NaOH) solution at 52 °C to generate that of carboxyl groups in the hydrogel. The carboxyl groups generated were chemically coupled with the amino groups of type I collagen to prepare the hydrogel with a concentration gradient of collagen immobilized. The attachment of L929 fibroblasts was evaluated for the collagen-immobilized hydrogel prepared. The amount gradient of carboxyl groups in the hydrogel increased with an increase in the NaOH concentration while the carboxyl groups gradient enabled to generate a gradient of collagen immobilized in the hydrogel. On the other hand, the number of fibroblasts adhered depended on the amount of collagen immobilized. These findings indicate that the adhesion behavior of cells is modified by the concentration gradient of biomolecule in the three-dimensional scaffold of cells. Molecular Diversity Preservation International 2010-03-26 /pmc/articles/PMC5445852/ http://dx.doi.org/10.3390/ma3042393 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Yamamoto, Masaya Yanase, Kaoru Tabata, Yasuhiko Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups |
title | Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups |
title_full | Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups |
title_fullStr | Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups |
title_full_unstemmed | Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups |
title_short | Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups |
title_sort | generation of type i collagen gradient in polyacrylamide hydrogels by a simple diffusion-controlled hydrolysis of amide groups |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445852/ http://dx.doi.org/10.3390/ma3042393 |
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