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Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups

The objective of this study is to develop an easy and simple diffusion-controlled fabrication technique to generate the concentration gradient of biomolecules in hydrogels. Polyacrylamide (PAAm) hydrogels with a concentration gradient of type I collagen were prepared to evaluate the cell adhesion. T...

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Detalles Bibliográficos
Autores principales: Yamamoto, Masaya, Yanase, Kaoru, Tabata, Yasuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445852/
http://dx.doi.org/10.3390/ma3042393
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author Yamamoto, Masaya
Yanase, Kaoru
Tabata, Yasuhiko
author_facet Yamamoto, Masaya
Yanase, Kaoru
Tabata, Yasuhiko
author_sort Yamamoto, Masaya
collection PubMed
description The objective of this study is to develop an easy and simple diffusion-controlled fabrication technique to generate the concentration gradient of biomolecules in hydrogels. Polyacrylamide (PAAm) hydrogels with a concentration gradient of type I collagen were prepared to evaluate the cell adhesion. The PAAm hydrogel was exposed to a gradient concentration of sodium hydroxide (NaOH) solution at 52 °C to generate that of carboxyl groups in the hydrogel. The carboxyl groups generated were chemically coupled with the amino groups of type I collagen to prepare the hydrogel with a concentration gradient of collagen immobilized. The attachment of L929 fibroblasts was evaluated for the collagen-immobilized hydrogel prepared. The amount gradient of carboxyl groups in the hydrogel increased with an increase in the NaOH concentration while the carboxyl groups gradient enabled to generate a gradient of collagen immobilized in the hydrogel. On the other hand, the number of fibroblasts adhered depended on the amount of collagen immobilized. These findings indicate that the adhesion behavior of cells is modified by the concentration gradient of biomolecule in the three-dimensional scaffold of cells.
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spelling pubmed-54458522017-07-28 Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups Yamamoto, Masaya Yanase, Kaoru Tabata, Yasuhiko Materials (Basel) Article The objective of this study is to develop an easy and simple diffusion-controlled fabrication technique to generate the concentration gradient of biomolecules in hydrogels. Polyacrylamide (PAAm) hydrogels with a concentration gradient of type I collagen were prepared to evaluate the cell adhesion. The PAAm hydrogel was exposed to a gradient concentration of sodium hydroxide (NaOH) solution at 52 °C to generate that of carboxyl groups in the hydrogel. The carboxyl groups generated were chemically coupled with the amino groups of type I collagen to prepare the hydrogel with a concentration gradient of collagen immobilized. The attachment of L929 fibroblasts was evaluated for the collagen-immobilized hydrogel prepared. The amount gradient of carboxyl groups in the hydrogel increased with an increase in the NaOH concentration while the carboxyl groups gradient enabled to generate a gradient of collagen immobilized in the hydrogel. On the other hand, the number of fibroblasts adhered depended on the amount of collagen immobilized. These findings indicate that the adhesion behavior of cells is modified by the concentration gradient of biomolecule in the three-dimensional scaffold of cells. Molecular Diversity Preservation International 2010-03-26 /pmc/articles/PMC5445852/ http://dx.doi.org/10.3390/ma3042393 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yamamoto, Masaya
Yanase, Kaoru
Tabata, Yasuhiko
Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups
title Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups
title_full Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups
title_fullStr Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups
title_full_unstemmed Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups
title_short Generation of Type I Collagen Gradient in Polyacrylamide Hydrogels by a Simple Diffusion-Controlled Hydrolysis of Amide Groups
title_sort generation of type i collagen gradient in polyacrylamide hydrogels by a simple diffusion-controlled hydrolysis of amide groups
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445852/
http://dx.doi.org/10.3390/ma3042393
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