Magnetic techniques for the isolation and purification of proteins and peptides

Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magne...

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Detalles Bibliográficos
Autores principales: Safarik, Ivo, Safarikova, Mirka
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544596/
https://www.ncbi.nlm.nih.gov/pubmed/15566570
http://dx.doi.org/10.1186/1477-044X-2-7
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author Safarik, Ivo
Safarikova, Mirka
author_facet Safarik, Ivo
Safarikova, Mirka
author_sort Safarik, Ivo
collection PubMed
description Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques.
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spelling pubmed-5445962005-01-16 Magnetic techniques for the isolation and purification of proteins and peptides Safarik, Ivo Safarikova, Mirka Biomagn Res Technol Review Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques. BioMed Central 2004-11-26 /pmc/articles/PMC544596/ /pubmed/15566570 http://dx.doi.org/10.1186/1477-044X-2-7 Text en Copyright © 2004 Safarik and Safarikova; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Safarik, Ivo
Safarikova, Mirka
Magnetic techniques for the isolation and purification of proteins and peptides
title Magnetic techniques for the isolation and purification of proteins and peptides
title_full Magnetic techniques for the isolation and purification of proteins and peptides
title_fullStr Magnetic techniques for the isolation and purification of proteins and peptides
title_full_unstemmed Magnetic techniques for the isolation and purification of proteins and peptides
title_short Magnetic techniques for the isolation and purification of proteins and peptides
title_sort magnetic techniques for the isolation and purification of proteins and peptides
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544596/
https://www.ncbi.nlm.nih.gov/pubmed/15566570
http://dx.doi.org/10.1186/1477-044X-2-7
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