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QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS
BACKGROUND: A simple and sensitive thin-layer chromatographic method has been established for quantification of glycyrrhizin in Glycyrrhiza glabra rhizome and baby herbal formulations by validated Reverse Phase HPTLC method. MATERIALS AND METHODS: RP-HPTLC Method was carried out using glass coated w...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
African Traditional Herbal Medicine Supporters Initiative (ATHMSI)
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446444/ https://www.ncbi.nlm.nih.gov/pubmed/28573236 http://dx.doi.org/10.21010/ajtcam.v14i2.21 |
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author | Alam, Prawez Foudah, Ahmed I. Zaatout, Hala H. T, Kamal Y Abdel-Kader, Maged S. |
author_facet | Alam, Prawez Foudah, Ahmed I. Zaatout, Hala H. T, Kamal Y Abdel-Kader, Maged S. |
author_sort | Alam, Prawez |
collection | PubMed |
description | BACKGROUND: A simple and sensitive thin-layer chromatographic method has been established for quantification of glycyrrhizin in Glycyrrhiza glabra rhizome and baby herbal formulations by validated Reverse Phase HPTLC method. MATERIALS AND METHODS: RP-HPTLC Method was carried out using glass coated with RP-18 silica gel 60 F254S HPTLC plates using methanol-water (7: 3 v/v) as mobile phase. RESULTS: The developed plate was scanned and quantified densitometrically at 256 nm. Glycyrrhizin peaks from Glycyrrhiza glabra rhizome and baby herbal formulations were identified by comparing their single spot at R(f) = 0.63 ± 0.01. Linear regression analysis revealed a good linear relationship between peak area and amount of glycyrrhizin in the range of 2000-7000 ng/band. CONCLUSION: The method was validated, in accordance with ICH guidelines for precision, accuracy, and robustness. The proposed method will be useful to enumerate the therapeutic dose of glycyrrhizin in herbal formulations as well as in bulk drug. |
format | Online Article Text |
id | pubmed-5446444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | African Traditional Herbal Medicine Supporters Initiative (ATHMSI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-54464442017-06-05 QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS Alam, Prawez Foudah, Ahmed I. Zaatout, Hala H. T, Kamal Y Abdel-Kader, Maged S. Afr J Tradit Complement Altern Med Article BACKGROUND: A simple and sensitive thin-layer chromatographic method has been established for quantification of glycyrrhizin in Glycyrrhiza glabra rhizome and baby herbal formulations by validated Reverse Phase HPTLC method. MATERIALS AND METHODS: RP-HPTLC Method was carried out using glass coated with RP-18 silica gel 60 F254S HPTLC plates using methanol-water (7: 3 v/v) as mobile phase. RESULTS: The developed plate was scanned and quantified densitometrically at 256 nm. Glycyrrhizin peaks from Glycyrrhiza glabra rhizome and baby herbal formulations were identified by comparing their single spot at R(f) = 0.63 ± 0.01. Linear regression analysis revealed a good linear relationship between peak area and amount of glycyrrhizin in the range of 2000-7000 ng/band. CONCLUSION: The method was validated, in accordance with ICH guidelines for precision, accuracy, and robustness. The proposed method will be useful to enumerate the therapeutic dose of glycyrrhizin in herbal formulations as well as in bulk drug. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2017-01-13 /pmc/articles/PMC5446444/ /pubmed/28573236 http://dx.doi.org/10.21010/ajtcam.v14i2.21 Text en Copyright: © 2017 Afr. J. Traditional Complementary and Alternative Medicines http://creativecommons.org/licenses/CC-BY/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License |
spellingShingle | Article Alam, Prawez Foudah, Ahmed I. Zaatout, Hala H. T, Kamal Y Abdel-Kader, Maged S. QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS |
title | QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS |
title_full | QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS |
title_fullStr | QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS |
title_full_unstemmed | QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS |
title_short | QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS |
title_sort | quantification of glycyrrhizin biomarker in glycyrrhiza glabra rhizome and baby herbal formulations by validated rp-hptlc methods |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446444/ https://www.ncbi.nlm.nih.gov/pubmed/28573236 http://dx.doi.org/10.21010/ajtcam.v14i2.21 |
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