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The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study

BACKGROUND: Chlormadinone acetate (CMA) is a derivative of progesterone and is used as an oral contraceptive. The aim of this study was to investigate the effects of CMA on odontogenic differentiation and mineralization of human dental pulp cells (hDPCs) and related signaling pathways. METHODS: Cell...

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Autores principales: Kim, Se-Min, Lee, Bin-Na, Koh, Jeong-Tae, Chang, Hoon-Sang, Hwang, In-Nam, Oh, Won-Mann, Min, Kyung-San, Hwang, Yun-Chan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446736/
https://www.ncbi.nlm.nih.gov/pubmed/28549486
http://dx.doi.org/10.1186/s12903-017-0379-0
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author Kim, Se-Min
Lee, Bin-Na
Koh, Jeong-Tae
Chang, Hoon-Sang
Hwang, In-Nam
Oh, Won-Mann
Min, Kyung-San
Hwang, Yun-Chan
author_facet Kim, Se-Min
Lee, Bin-Na
Koh, Jeong-Tae
Chang, Hoon-Sang
Hwang, In-Nam
Oh, Won-Mann
Min, Kyung-San
Hwang, Yun-Chan
author_sort Kim, Se-Min
collection PubMed
description BACKGROUND: Chlormadinone acetate (CMA) is a derivative of progesterone and is used as an oral contraceptive. The aim of this study was to investigate the effects of CMA on odontogenic differentiation and mineralization of human dental pulp cells (hDPCs) and related signaling pathways. METHODS: Cell viability was determined by the water-soluble tetrazolium (WST)-1 assay. Odontogenic differentiation of hDPCs was evaluated by real-time polymerase chain reaction using odontogenic marker genes, such as alkaline phosphatase (ALP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP), and dentin matrix protein-1 (DMP-1). Mineralization of hDPCs was evaluated by ALP staining and alizarin red staining. The extracellular signal-regulated kinase (ERK) pathway was examined by Western blot analysis. RESULTS: There was no statistically significant difference in cell viability between the control and CMA-treated groups. Our analysis of odontogenic marker genes indicated that CMA enhanced the expression of those genes. CMA-treated hDPCs showed increased ALP activity and formation of mineralized nodules, compared with control-treated cells. In addition, CMA stimulation resulted in phosphorylation of ERK and resulted in inhibition of downstream molecules by the ERK inhibitor U0126. CONCLUSIONS: These findings suggest that CMA improves odontogenic differentiation and mineralization of hDPCs through the ERK signaling pathway.
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spelling pubmed-54467362017-05-30 The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study Kim, Se-Min Lee, Bin-Na Koh, Jeong-Tae Chang, Hoon-Sang Hwang, In-Nam Oh, Won-Mann Min, Kyung-San Hwang, Yun-Chan BMC Oral Health Research Article BACKGROUND: Chlormadinone acetate (CMA) is a derivative of progesterone and is used as an oral contraceptive. The aim of this study was to investigate the effects of CMA on odontogenic differentiation and mineralization of human dental pulp cells (hDPCs) and related signaling pathways. METHODS: Cell viability was determined by the water-soluble tetrazolium (WST)-1 assay. Odontogenic differentiation of hDPCs was evaluated by real-time polymerase chain reaction using odontogenic marker genes, such as alkaline phosphatase (ALP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP), and dentin matrix protein-1 (DMP-1). Mineralization of hDPCs was evaluated by ALP staining and alizarin red staining. The extracellular signal-regulated kinase (ERK) pathway was examined by Western blot analysis. RESULTS: There was no statistically significant difference in cell viability between the control and CMA-treated groups. Our analysis of odontogenic marker genes indicated that CMA enhanced the expression of those genes. CMA-treated hDPCs showed increased ALP activity and formation of mineralized nodules, compared with control-treated cells. In addition, CMA stimulation resulted in phosphorylation of ERK and resulted in inhibition of downstream molecules by the ERK inhibitor U0126. CONCLUSIONS: These findings suggest that CMA improves odontogenic differentiation and mineralization of hDPCs through the ERK signaling pathway. BioMed Central 2017-05-26 /pmc/articles/PMC5446736/ /pubmed/28549486 http://dx.doi.org/10.1186/s12903-017-0379-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kim, Se-Min
Lee, Bin-Na
Koh, Jeong-Tae
Chang, Hoon-Sang
Hwang, In-Nam
Oh, Won-Mann
Min, Kyung-San
Hwang, Yun-Chan
The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
title The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
title_full The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
title_fullStr The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
title_full_unstemmed The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
title_short The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
title_sort effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446736/
https://www.ncbi.nlm.nih.gov/pubmed/28549486
http://dx.doi.org/10.1186/s12903-017-0379-0
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