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p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats

BACKGROUND: The 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p’-DDT) is a known persistent organic pollutant and male reproductive toxicant. The present study is designed to test the hypothesis that oxidative stress mediates p,p’-DDT-induced apoptosis in testis. METHODS: Male Wistar rats receive...

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Autores principales: Marouani, Neila, Hallegue, Dorsaf, Sakly, Mohsen, Benkhalifa, Moncef, Ben Rhouma, Khémais, Tebourbi, Olfa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446748/
https://www.ncbi.nlm.nih.gov/pubmed/28549437
http://dx.doi.org/10.1186/s12958-017-0259-0
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author Marouani, Neila
Hallegue, Dorsaf
Sakly, Mohsen
Benkhalifa, Moncef
Ben Rhouma, Khémais
Tebourbi, Olfa
author_facet Marouani, Neila
Hallegue, Dorsaf
Sakly, Mohsen
Benkhalifa, Moncef
Ben Rhouma, Khémais
Tebourbi, Olfa
author_sort Marouani, Neila
collection PubMed
description BACKGROUND: The 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p’-DDT) is a known persistent organic pollutant and male reproductive toxicant. The present study is designed to test the hypothesis that oxidative stress mediates p,p’-DDT-induced apoptosis in testis. METHODS: Male Wistar rats received an intraperitoneal (ip) injection of the pesticide at doses of 50 and 100mg/kg for 10 consecutive days. The oxidative stress was evaluated by biomarkers such lipid peroxidation (LPO) and metallothioneins (MTs) levels. Antioxidant enzymes activities was assessed by determination of superoxide dismutase (SOD), catalase (CAT) and hydrogen peroxide (H(2)O(2)) production. In addition, glutathione-dependent enzymes and reducing power in testis was evaluated by glutathione peroxidase (Gpx), glutathione reductase (GR), glutathione S-transferase (GST) activities and reduced and oxidized glutathione (GSH - GSSG) levels. Apoptosis was evaluated by DNA fragmentation detected by agarose gel electrophoresis. Germinal cells apoptosis and the apoptotic index was assessed through the TUNEL assay. RESULTS: After 10 days of treatment, an increase in LPO level and H(2)O(2) production occurred, while MTs level, SOD and CAT activities were decreased. Also, the Gpx, GR, GST, and GSH activities were decreased, whereas GSSG activity was increased. Testicular tissues of treated rats showed pronounced degradation of the DNA into oligonucleotides as seen in the typical electrophoretic DNA ladder pattern. Intense apoptosis was observed in germinal cells of DDT-exposed rats. In addition, the apoptotic index was significantly increased in testis of DDT-treated rats. CONCLUSIONS: These results clearly suggest that DDT sub-acute treatment causes oxidative stress in rat testis leading to apoptosis.
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spelling pubmed-54467482017-05-30 p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats Marouani, Neila Hallegue, Dorsaf Sakly, Mohsen Benkhalifa, Moncef Ben Rhouma, Khémais Tebourbi, Olfa Reprod Biol Endocrinol Research BACKGROUND: The 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p’-DDT) is a known persistent organic pollutant and male reproductive toxicant. The present study is designed to test the hypothesis that oxidative stress mediates p,p’-DDT-induced apoptosis in testis. METHODS: Male Wistar rats received an intraperitoneal (ip) injection of the pesticide at doses of 50 and 100mg/kg for 10 consecutive days. The oxidative stress was evaluated by biomarkers such lipid peroxidation (LPO) and metallothioneins (MTs) levels. Antioxidant enzymes activities was assessed by determination of superoxide dismutase (SOD), catalase (CAT) and hydrogen peroxide (H(2)O(2)) production. In addition, glutathione-dependent enzymes and reducing power in testis was evaluated by glutathione peroxidase (Gpx), glutathione reductase (GR), glutathione S-transferase (GST) activities and reduced and oxidized glutathione (GSH - GSSG) levels. Apoptosis was evaluated by DNA fragmentation detected by agarose gel electrophoresis. Germinal cells apoptosis and the apoptotic index was assessed through the TUNEL assay. RESULTS: After 10 days of treatment, an increase in LPO level and H(2)O(2) production occurred, while MTs level, SOD and CAT activities were decreased. Also, the Gpx, GR, GST, and GSH activities were decreased, whereas GSSG activity was increased. Testicular tissues of treated rats showed pronounced degradation of the DNA into oligonucleotides as seen in the typical electrophoretic DNA ladder pattern. Intense apoptosis was observed in germinal cells of DDT-exposed rats. In addition, the apoptotic index was significantly increased in testis of DDT-treated rats. CONCLUSIONS: These results clearly suggest that DDT sub-acute treatment causes oxidative stress in rat testis leading to apoptosis. BioMed Central 2017-05-26 /pmc/articles/PMC5446748/ /pubmed/28549437 http://dx.doi.org/10.1186/s12958-017-0259-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Marouani, Neila
Hallegue, Dorsaf
Sakly, Mohsen
Benkhalifa, Moncef
Ben Rhouma, Khémais
Tebourbi, Olfa
p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats
title p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats
title_full p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats
title_fullStr p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats
title_full_unstemmed p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats
title_short p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats
title_sort p,p’-ddt induces testicular oxidative stress-induced apoptosis in adult rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446748/
https://www.ncbi.nlm.nih.gov/pubmed/28549437
http://dx.doi.org/10.1186/s12958-017-0259-0
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