Optimization of Aflatoxin B1 Aptasensing

Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. De...

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Autores principales: Jafari, Marzieh, Rezaei, Mohsen, Kalantari, Heibatullah, Tabarzad, Maryam, Daraei, Bahram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446859/
https://www.ncbi.nlm.nih.gov/pubmed/28588614
http://dx.doi.org/10.1155/2017/2461354
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author Jafari, Marzieh
Rezaei, Mohsen
Kalantari, Heibatullah
Tabarzad, Maryam
Daraei, Bahram
author_facet Jafari, Marzieh
Rezaei, Mohsen
Kalantari, Heibatullah
Tabarzad, Maryam
Daraei, Bahram
author_sort Jafari, Marzieh
collection PubMed
description Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. Detecting approach is based on the binding affinity of aflatoxin B1 to its specific aptamer and conversion of substrate to a detectable colorimetric signal by a linked DNAzyme. Compared to conventional methods for aflatoxin B1 detection, DNA-based assay has the advantages of low cost, long-term stability, and rapid, simple, and user-friendly steps.
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spelling pubmed-54468592017-06-06 Optimization of Aflatoxin B1 Aptasensing Jafari, Marzieh Rezaei, Mohsen Kalantari, Heibatullah Tabarzad, Maryam Daraei, Bahram J Toxicol Research Article Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. Detecting approach is based on the binding affinity of aflatoxin B1 to its specific aptamer and conversion of substrate to a detectable colorimetric signal by a linked DNAzyme. Compared to conventional methods for aflatoxin B1 detection, DNA-based assay has the advantages of low cost, long-term stability, and rapid, simple, and user-friendly steps. Hindawi 2017 2017-05-14 /pmc/articles/PMC5446859/ /pubmed/28588614 http://dx.doi.org/10.1155/2017/2461354 Text en Copyright © 2017 Marzieh Jafari et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jafari, Marzieh
Rezaei, Mohsen
Kalantari, Heibatullah
Tabarzad, Maryam
Daraei, Bahram
Optimization of Aflatoxin B1 Aptasensing
title Optimization of Aflatoxin B1 Aptasensing
title_full Optimization of Aflatoxin B1 Aptasensing
title_fullStr Optimization of Aflatoxin B1 Aptasensing
title_full_unstemmed Optimization of Aflatoxin B1 Aptasensing
title_short Optimization of Aflatoxin B1 Aptasensing
title_sort optimization of aflatoxin b1 aptasensing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446859/
https://www.ncbi.nlm.nih.gov/pubmed/28588614
http://dx.doi.org/10.1155/2017/2461354
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