Gene and protein expression of a soluble form of CTLA-4 in a healthy dog

Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) wa...

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Autores principales: TAGAWA, Michihito, YAMAMOTO, Yuhei, SHIMBO, Genya, IGUCHI, Aiko, XUAN, Xuenan, TOMIHARI, Mizuki, MIYAHARA, Kazuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5447975/
https://www.ncbi.nlm.nih.gov/pubmed/28392507
http://dx.doi.org/10.1292/jvms.16-0583
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author TAGAWA, Michihito
YAMAMOTO, Yuhei
SHIMBO, Genya
IGUCHI, Aiko
XUAN, Xuenan
TOMIHARI, Mizuki
MIYAHARA, Kazuro
author_facet TAGAWA, Michihito
YAMAMOTO, Yuhei
SHIMBO, Genya
IGUCHI, Aiko
XUAN, Xuenan
TOMIHARI, Mizuki
MIYAHARA, Kazuro
author_sort TAGAWA, Michihito
collection PubMed
description Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) was detected in normal human serum. We describe alternatively spliced mRNA expressed in peripheral blood mononuclear cells obtained from a healthy dog lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study.
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spelling pubmed-54479752017-06-01 Gene and protein expression of a soluble form of CTLA-4 in a healthy dog TAGAWA, Michihito YAMAMOTO, Yuhei SHIMBO, Genya IGUCHI, Aiko XUAN, Xuenan TOMIHARI, Mizuki MIYAHARA, Kazuro J Vet Med Sci Immunology Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) was detected in normal human serum. We describe alternatively spliced mRNA expressed in peripheral blood mononuclear cells obtained from a healthy dog lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study. The Japanese Society of Veterinary Science 2017-04-07 2017-05 /pmc/articles/PMC5447975/ /pubmed/28392507 http://dx.doi.org/10.1292/jvms.16-0583 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Immunology
TAGAWA, Michihito
YAMAMOTO, Yuhei
SHIMBO, Genya
IGUCHI, Aiko
XUAN, Xuenan
TOMIHARI, Mizuki
MIYAHARA, Kazuro
Gene and protein expression of a soluble form of CTLA-4 in a healthy dog
title Gene and protein expression of a soluble form of CTLA-4 in a healthy dog
title_full Gene and protein expression of a soluble form of CTLA-4 in a healthy dog
title_fullStr Gene and protein expression of a soluble form of CTLA-4 in a healthy dog
title_full_unstemmed Gene and protein expression of a soluble form of CTLA-4 in a healthy dog
title_short Gene and protein expression of a soluble form of CTLA-4 in a healthy dog
title_sort gene and protein expression of a soluble form of ctla-4 in a healthy dog
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5447975/
https://www.ncbi.nlm.nih.gov/pubmed/28392507
http://dx.doi.org/10.1292/jvms.16-0583
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