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Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity
Hyperoxaluria and oxidative stress are risk factors in calcium oxalate (CaOx) stone formation. Supplement with antioxidant could be effective in prevention of recurrent stone formation. The present study aims to evaluate the protective effects of vitamin E and vitamin C in hyperoxaluric rat. The exp...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5447979/ https://www.ncbi.nlm.nih.gov/pubmed/28392511 http://dx.doi.org/10.1292/jvms.17-0083 |
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author | JATURAKAN, Orapun DISSAYABUTRA, Thasinas CHAIYABUTR, Narongsak KIJTAWORNRAT, Anusak TOSUKHOWONG, Piyaratana RUNGSIPIPAT, Anudep NHUJAK, Thumnoon BURANAKARL, Chollada |
author_facet | JATURAKAN, Orapun DISSAYABUTRA, Thasinas CHAIYABUTR, Narongsak KIJTAWORNRAT, Anusak TOSUKHOWONG, Piyaratana RUNGSIPIPAT, Anudep NHUJAK, Thumnoon BURANAKARL, Chollada |
author_sort | JATURAKAN, Orapun |
collection | PubMed |
description | Hyperoxaluria and oxidative stress are risk factors in calcium oxalate (CaOx) stone formation. Supplement with antioxidant could be effective in prevention of recurrent stone formation. The present study aims to evaluate the protective effects of vitamin E and vitamin C in hyperoxaluric rat. The experiment was performed in rats for 21 days. Rats were divided into 5 groups as follows: control (group 1, n=8), hyperoxaluric rats (group 2, n=8), hyperoxaluric rats with vitamin E supplement (group 3, n=7), hyperoxaluric rats with vitamin C supplement (group 4, n=7) and hyperoxaluric rats with vitamin E and C supplement (group 5, n=7). Hyperoxaluria was induced by feeding hydroxyl L-proline (HLP) 2% w/v dissolved in drinking water. Intraperitoneal 200 mg/kg of vitamin E was given in groups 3 and 5 on days 1, 6, 11 and 16, while 500 mg of vitamin C was injected intravenously in groups 4 and 5 on days 1 and 11. Renal functions and oxidative status were measured. The urinary oxalate excretion was increased in HLP supplement rats, while glomerular filtration rate, proximal water and sodium reabsorption were significantly lower in group 2 compared with a control (P<0.05). Giving antioxidants significantly lower urinary calcium oxalate crystals (P<0.05). Hyperoxaluric rats had higher plasma malondialdehyde (PMDA) and lower urinary total antioxidant status (UTAS), which were alleviated by vitamin E and/or vitamin C supplement. In conclusion, giving combination of vitamin E and vitamin C exerts a protective role against HLP-induced oxalate nephropathy. |
format | Online Article Text |
id | pubmed-5447979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54479792017-06-01 Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity JATURAKAN, Orapun DISSAYABUTRA, Thasinas CHAIYABUTR, Narongsak KIJTAWORNRAT, Anusak TOSUKHOWONG, Piyaratana RUNGSIPIPAT, Anudep NHUJAK, Thumnoon BURANAKARL, Chollada J Vet Med Sci Physiology Hyperoxaluria and oxidative stress are risk factors in calcium oxalate (CaOx) stone formation. Supplement with antioxidant could be effective in prevention of recurrent stone formation. The present study aims to evaluate the protective effects of vitamin E and vitamin C in hyperoxaluric rat. The experiment was performed in rats for 21 days. Rats were divided into 5 groups as follows: control (group 1, n=8), hyperoxaluric rats (group 2, n=8), hyperoxaluric rats with vitamin E supplement (group 3, n=7), hyperoxaluric rats with vitamin C supplement (group 4, n=7) and hyperoxaluric rats with vitamin E and C supplement (group 5, n=7). Hyperoxaluria was induced by feeding hydroxyl L-proline (HLP) 2% w/v dissolved in drinking water. Intraperitoneal 200 mg/kg of vitamin E was given in groups 3 and 5 on days 1, 6, 11 and 16, while 500 mg of vitamin C was injected intravenously in groups 4 and 5 on days 1 and 11. Renal functions and oxidative status were measured. The urinary oxalate excretion was increased in HLP supplement rats, while glomerular filtration rate, proximal water and sodium reabsorption were significantly lower in group 2 compared with a control (P<0.05). Giving antioxidants significantly lower urinary calcium oxalate crystals (P<0.05). Hyperoxaluric rats had higher plasma malondialdehyde (PMDA) and lower urinary total antioxidant status (UTAS), which were alleviated by vitamin E and/or vitamin C supplement. In conclusion, giving combination of vitamin E and vitamin C exerts a protective role against HLP-induced oxalate nephropathy. The Japanese Society of Veterinary Science 2017-04-08 2017-05 /pmc/articles/PMC5447979/ /pubmed/28392511 http://dx.doi.org/10.1292/jvms.17-0083 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Physiology JATURAKAN, Orapun DISSAYABUTRA, Thasinas CHAIYABUTR, Narongsak KIJTAWORNRAT, Anusak TOSUKHOWONG, Piyaratana RUNGSIPIPAT, Anudep NHUJAK, Thumnoon BURANAKARL, Chollada Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity |
title | Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity |
title_full | Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity |
title_fullStr | Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity |
title_full_unstemmed | Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity |
title_short | Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity |
title_sort | combination of vitamin e and vitamin c alleviates renal function in hyperoxaluric rats via antioxidant activity |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5447979/ https://www.ncbi.nlm.nih.gov/pubmed/28392511 http://dx.doi.org/10.1292/jvms.17-0083 |
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