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ANRIL Genetic Variants in Iranian Breast Cancer Patients
OBJECTIVE: The genetic variants of the long non-coding RNA ANRIL (an antisense noncoding RNA in the INK4 locus) as well as its expression have been shown to be associated with several human diseases including cancers. The aim of this study was to examine the association of ANRIL variants with breast...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448323/ https://www.ncbi.nlm.nih.gov/pubmed/28580310 http://dx.doi.org/10.22074/cellj.2017.4496 |
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author | Khorshidi, Hamid Reza Taheri, Mohammad Noroozi, Rezvan Sarrafzadeh, Shaghayegh Sayad, Arezou Ghafouri-Fard, Soudeh |
author_facet | Khorshidi, Hamid Reza Taheri, Mohammad Noroozi, Rezvan Sarrafzadeh, Shaghayegh Sayad, Arezou Ghafouri-Fard, Soudeh |
author_sort | Khorshidi, Hamid Reza |
collection | PubMed |
description | OBJECTIVE: The genetic variants of the long non-coding RNA ANRIL (an antisense noncoding RNA in the INK4 locus) as well as its expression have been shown to be associated with several human diseases including cancers. The aim of this study was to examine the association of ANRIL variants with breast cancer susceptibility in Iranian patients. MATERIALS AND METHODS: In this case-control study, we genotyped rs1333045, rs4977574, rs1333048 and rs10757278 single nucleotide polymorphisms (SNPs) in 122 breast can- cer patients as well as in 200 normal age-matched subjects by tetra-primer amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR). RESULTS: The TT genotype at rs1333045 was significantly over-represented among pa- tients (P=0.038) but did not remain significant after multiple-testing correction. In addi- tion, among all observed haplotypes (with SNP order of rs1333045, rs1333048 rs4977574 and rs10757278), four haplotypes were shown to be associated with breast cancer risk. However, after multiple testing corrections, TCGA was the only haplotype which remained significant. CONCLUSION: These results suggest that breast cancer risk is significantly associated with ANRIL variants. Future work analyzing the expression of different associated ANRIL haplotypes would further shed light on the role of ANRIL in this disease. |
format | Online Article Text |
id | pubmed-5448323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-54483232017-06-02 ANRIL Genetic Variants in Iranian Breast Cancer Patients Khorshidi, Hamid Reza Taheri, Mohammad Noroozi, Rezvan Sarrafzadeh, Shaghayegh Sayad, Arezou Ghafouri-Fard, Soudeh Cell J Original Article OBJECTIVE: The genetic variants of the long non-coding RNA ANRIL (an antisense noncoding RNA in the INK4 locus) as well as its expression have been shown to be associated with several human diseases including cancers. The aim of this study was to examine the association of ANRIL variants with breast cancer susceptibility in Iranian patients. MATERIALS AND METHODS: In this case-control study, we genotyped rs1333045, rs4977574, rs1333048 and rs10757278 single nucleotide polymorphisms (SNPs) in 122 breast can- cer patients as well as in 200 normal age-matched subjects by tetra-primer amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR). RESULTS: The TT genotype at rs1333045 was significantly over-represented among pa- tients (P=0.038) but did not remain significant after multiple-testing correction. In addi- tion, among all observed haplotypes (with SNP order of rs1333045, rs1333048 rs4977574 and rs10757278), four haplotypes were shown to be associated with breast cancer risk. However, after multiple testing corrections, TCGA was the only haplotype which remained significant. CONCLUSION: These results suggest that breast cancer risk is significantly associated with ANRIL variants. Future work analyzing the expression of different associated ANRIL haplotypes would further shed light on the role of ANRIL in this disease. Royan Institute 2017 2017-05-17 /pmc/articles/PMC5448323/ /pubmed/28580310 http://dx.doi.org/10.22074/cellj.2017.4496 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Khorshidi, Hamid Reza Taheri, Mohammad Noroozi, Rezvan Sarrafzadeh, Shaghayegh Sayad, Arezou Ghafouri-Fard, Soudeh ANRIL Genetic Variants in Iranian Breast Cancer Patients |
title | ANRIL Genetic Variants in Iranian Breast Cancer Patients |
title_full | ANRIL Genetic Variants in Iranian Breast Cancer Patients |
title_fullStr | ANRIL Genetic Variants in Iranian Breast Cancer Patients |
title_full_unstemmed | ANRIL Genetic Variants in Iranian Breast Cancer Patients |
title_short | ANRIL Genetic Variants in Iranian Breast Cancer Patients |
title_sort | anril genetic variants in iranian breast cancer patients |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448323/ https://www.ncbi.nlm.nih.gov/pubmed/28580310 http://dx.doi.org/10.22074/cellj.2017.4496 |
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