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Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering
This research activity was aimed at the development of dual-scale scaffolds consisting of three-dimensional constructs of aligned poly(ε-caprolactone) (PCL) microfilaments and electrospun poly(lactic-co-glycolic acid) (PLGA) fibers. PCL constructs composed by layers of parallel microsized filaments...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448499/ https://www.ncbi.nlm.nih.gov/pubmed/28880003 http://dx.doi.org/10.3390/ma4030527 |
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author | Mota, Carlos Puppi, Dario Dinucci, Dinuccio Errico, Cesare Bártolo, Paulo Chiellini, Federica |
author_facet | Mota, Carlos Puppi, Dario Dinucci, Dinuccio Errico, Cesare Bártolo, Paulo Chiellini, Federica |
author_sort | Mota, Carlos |
collection | PubMed |
description | This research activity was aimed at the development of dual-scale scaffolds consisting of three-dimensional constructs of aligned poly(ε-caprolactone) (PCL) microfilaments and electrospun poly(lactic-co-glycolic acid) (PLGA) fibers. PCL constructs composed by layers of parallel microsized filaments (0/90° lay-down pattern), with a diameter of around 365 μm and interfilament distance of around 191 μm, were produced using a melt extrusion-based additive manufacturing technique. PLGA electrospun fibers with a diameter of around 1 μm were collected on top of the PCL constructs with different thicknesses, showing a certain degree of alignment. Cell culture experiments employing the MC3T3 murine preosteoblast cell line showed good cell viability and adhesion on the dual-scale scaffolds. In particular, the influence of electrospun fibers on cell morphology and behavior was evident, as well as in creating a structural bridging for cell colonization in the interfilament gap. |
format | Online Article Text |
id | pubmed-5448499 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54484992017-07-28 Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering Mota, Carlos Puppi, Dario Dinucci, Dinuccio Errico, Cesare Bártolo, Paulo Chiellini, Federica Materials (Basel) Article This research activity was aimed at the development of dual-scale scaffolds consisting of three-dimensional constructs of aligned poly(ε-caprolactone) (PCL) microfilaments and electrospun poly(lactic-co-glycolic acid) (PLGA) fibers. PCL constructs composed by layers of parallel microsized filaments (0/90° lay-down pattern), with a diameter of around 365 μm and interfilament distance of around 191 μm, were produced using a melt extrusion-based additive manufacturing technique. PLGA electrospun fibers with a diameter of around 1 μm were collected on top of the PCL constructs with different thicknesses, showing a certain degree of alignment. Cell culture experiments employing the MC3T3 murine preosteoblast cell line showed good cell viability and adhesion on the dual-scale scaffolds. In particular, the influence of electrospun fibers on cell morphology and behavior was evident, as well as in creating a structural bridging for cell colonization in the interfilament gap. MDPI 2011-03-01 /pmc/articles/PMC5448499/ /pubmed/28880003 http://dx.doi.org/10.3390/ma4030527 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Mota, Carlos Puppi, Dario Dinucci, Dinuccio Errico, Cesare Bártolo, Paulo Chiellini, Federica Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering |
title | Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering |
title_full | Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering |
title_fullStr | Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering |
title_full_unstemmed | Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering |
title_short | Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering |
title_sort | dual-scale polymeric constructs as scaffolds for tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448499/ https://www.ncbi.nlm.nih.gov/pubmed/28880003 http://dx.doi.org/10.3390/ma4030527 |
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