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Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds

Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentrati...

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Autores principales: Borisjuk, Ljudmilla, Rolletschek, Hardy, Fuchs, Johannes, Melkus, Gerd, Neuberger, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448675/
https://www.ncbi.nlm.nih.gov/pubmed/28824152
http://dx.doi.org/10.3390/ma4081426
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author Borisjuk, Ljudmilla
Rolletschek, Hardy
Fuchs, Johannes
Melkus, Gerd
Neuberger, Thomas
author_facet Borisjuk, Ljudmilla
Rolletschek, Hardy
Fuchs, Johannes
Melkus, Gerd
Neuberger, Thomas
author_sort Borisjuk, Ljudmilla
collection PubMed
description Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology.
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spelling pubmed-54486752017-07-28 Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds Borisjuk, Ljudmilla Rolletschek, Hardy Fuchs, Johannes Melkus, Gerd Neuberger, Thomas Materials (Basel) Article Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology. MDPI 2011-08-16 /pmc/articles/PMC5448675/ /pubmed/28824152 http://dx.doi.org/10.3390/ma4081426 Text en © 2011 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Borisjuk, Ljudmilla
Rolletschek, Hardy
Fuchs, Johannes
Melkus, Gerd
Neuberger, Thomas
Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds
title Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds
title_full Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds
title_fullStr Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds
title_full_unstemmed Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds
title_short Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds
title_sort low and high field magnetic resonance for in vivo analysis of seeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448675/
https://www.ncbi.nlm.nih.gov/pubmed/28824152
http://dx.doi.org/10.3390/ma4081426
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