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Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds
Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentrati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448675/ https://www.ncbi.nlm.nih.gov/pubmed/28824152 http://dx.doi.org/10.3390/ma4081426 |
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author | Borisjuk, Ljudmilla Rolletschek, Hardy Fuchs, Johannes Melkus, Gerd Neuberger, Thomas |
author_facet | Borisjuk, Ljudmilla Rolletschek, Hardy Fuchs, Johannes Melkus, Gerd Neuberger, Thomas |
author_sort | Borisjuk, Ljudmilla |
collection | PubMed |
description | Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology. |
format | Online Article Text |
id | pubmed-5448675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54486752017-07-28 Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds Borisjuk, Ljudmilla Rolletschek, Hardy Fuchs, Johannes Melkus, Gerd Neuberger, Thomas Materials (Basel) Article Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology. MDPI 2011-08-16 /pmc/articles/PMC5448675/ /pubmed/28824152 http://dx.doi.org/10.3390/ma4081426 Text en © 2011 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Borisjuk, Ljudmilla Rolletschek, Hardy Fuchs, Johannes Melkus, Gerd Neuberger, Thomas Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds |
title | Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds |
title_full | Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds |
title_fullStr | Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds |
title_full_unstemmed | Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds |
title_short | Low and High Field Magnetic Resonance for in Vivo Analysis of Seeds |
title_sort | low and high field magnetic resonance for in vivo analysis of seeds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448675/ https://www.ncbi.nlm.nih.gov/pubmed/28824152 http://dx.doi.org/10.3390/ma4081426 |
work_keys_str_mv | AT borisjukljudmilla lowandhighfieldmagneticresonanceforinvivoanalysisofseeds AT rolletschekhardy lowandhighfieldmagneticresonanceforinvivoanalysisofseeds AT fuchsjohannes lowandhighfieldmagneticresonanceforinvivoanalysisofseeds AT melkusgerd lowandhighfieldmagneticresonanceforinvivoanalysisofseeds AT neubergerthomas lowandhighfieldmagneticresonanceforinvivoanalysisofseeds |