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Influence of Surface Processing on the Biocompatibility of Titanium

Surface conditioning of titanium middle ear implants results in an improved biocompatibility, which can be characterized by the properties of fibroblasts cultured on conditioned surfaces. Titanium has been established as a favorable biomaterial in ossicular chain reconstruction. The epithelization o...

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Autores principales: Wirsching, Kornelia, Lehle, Karla, Jacob, Peter, Gleich, Otto, Strutz, Jürgen, Kwok, Pingling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448690/
https://www.ncbi.nlm.nih.gov/pubmed/28824139
http://dx.doi.org/10.3390/ma4071238
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author Wirsching, Kornelia
Lehle, Karla
Jacob, Peter
Gleich, Otto
Strutz, Jürgen
Kwok, Pingling
author_facet Wirsching, Kornelia
Lehle, Karla
Jacob, Peter
Gleich, Otto
Strutz, Jürgen
Kwok, Pingling
author_sort Wirsching, Kornelia
collection PubMed
description Surface conditioning of titanium middle ear implants results in an improved biocompatibility, which can be characterized by the properties of fibroblasts cultured on conditioned surfaces. Titanium has been established as a favorable biomaterial in ossicular chain reconstruction. The epithelization of the surface of the implants is important for their integration and stable positioning in the middle ear. Mouse fibroblast cells were cultured on platelets made from pure Grade 2 titanium. Platelets that had been etched along their production process were compared to unetched platelets. The DNA in the cell nuclei was stained with DAPI and the actin filaments of the cytoskeleton were stained with FITC-conjugated phalloidin in order to analyze the cells grown on etched and unetched platelets by fluorescence microscopy. SEM (scanning electron microscopic) images were used to compare the surface structure of etched and unetched titanium platelets. There was a statistically significant increase of the area covered by the cytoplasm and increased actin expression by fibroblasts grown on the etched titanium platelets. In addition, the area of the platelets covered by nuclei on the etched platelets exceeded on average the one on unetched platelets, although this difference was not significant. The SEM pictures comparing unetched and etched titanium platelets showed a clear difference in surface structure. Surface conditioning of titanium implants improved the epithelization by fibroblasts and consequently etched titanium should be the preferred biomaterial for reconstructive middle ear surgery.
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spelling pubmed-54486902017-07-28 Influence of Surface Processing on the Biocompatibility of Titanium Wirsching, Kornelia Lehle, Karla Jacob, Peter Gleich, Otto Strutz, Jürgen Kwok, Pingling Materials (Basel) Article Surface conditioning of titanium middle ear implants results in an improved biocompatibility, which can be characterized by the properties of fibroblasts cultured on conditioned surfaces. Titanium has been established as a favorable biomaterial in ossicular chain reconstruction. The epithelization of the surface of the implants is important for their integration and stable positioning in the middle ear. Mouse fibroblast cells were cultured on platelets made from pure Grade 2 titanium. Platelets that had been etched along their production process were compared to unetched platelets. The DNA in the cell nuclei was stained with DAPI and the actin filaments of the cytoskeleton were stained with FITC-conjugated phalloidin in order to analyze the cells grown on etched and unetched platelets by fluorescence microscopy. SEM (scanning electron microscopic) images were used to compare the surface structure of etched and unetched titanium platelets. There was a statistically significant increase of the area covered by the cytoplasm and increased actin expression by fibroblasts grown on the etched titanium platelets. In addition, the area of the platelets covered by nuclei on the etched platelets exceeded on average the one on unetched platelets, although this difference was not significant. The SEM pictures comparing unetched and etched titanium platelets showed a clear difference in surface structure. Surface conditioning of titanium implants improved the epithelization by fibroblasts and consequently etched titanium should be the preferred biomaterial for reconstructive middle ear surgery. MDPI 2011-07-06 /pmc/articles/PMC5448690/ /pubmed/28824139 http://dx.doi.org/10.3390/ma4071238 Text en © 2011 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wirsching, Kornelia
Lehle, Karla
Jacob, Peter
Gleich, Otto
Strutz, Jürgen
Kwok, Pingling
Influence of Surface Processing on the Biocompatibility of Titanium
title Influence of Surface Processing on the Biocompatibility of Titanium
title_full Influence of Surface Processing on the Biocompatibility of Titanium
title_fullStr Influence of Surface Processing on the Biocompatibility of Titanium
title_full_unstemmed Influence of Surface Processing on the Biocompatibility of Titanium
title_short Influence of Surface Processing on the Biocompatibility of Titanium
title_sort influence of surface processing on the biocompatibility of titanium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448690/
https://www.ncbi.nlm.nih.gov/pubmed/28824139
http://dx.doi.org/10.3390/ma4071238
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