Cargando…

The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine

BACKGROUND: Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-documented phenomenon of an unknown mechanism. We have previously shown that the polymerase delta proofreading activity controls spontaneous mutagenesis in cells starved for histidine. To obt...

Descripción completa

Detalles Bibliográficos
Autores principales: Achilli, Alessandro, Matmati, Nabil, Casalone, Enrico, Morpurgo, Giorgio, Lucaccioni, Angela, Pavlov, Youri I, Babudri, Nora
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544876/
https://www.ncbi.nlm.nih.gov/pubmed/15617571
http://dx.doi.org/10.1186/1471-2156-5-34
_version_ 1782122171141718016
author Achilli, Alessandro
Matmati, Nabil
Casalone, Enrico
Morpurgo, Giorgio
Lucaccioni, Angela
Pavlov, Youri I
Babudri, Nora
author_facet Achilli, Alessandro
Matmati, Nabil
Casalone, Enrico
Morpurgo, Giorgio
Lucaccioni, Angela
Pavlov, Youri I
Babudri, Nora
author_sort Achilli, Alessandro
collection PubMed
description BACKGROUND: Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-documented phenomenon of an unknown mechanism. We have previously shown that the polymerase delta proofreading activity controls spontaneous mutagenesis in cells starved for histidine. To obtain further information, we compared the effect of adenine starvation on mutagenesis in wild-type cells and, in cells lacking the proofreading activity of polymerase delta (phenotype Exo(-), mutation pol3-01). RESULTS: Ade(+ )revertants accumulated at a very high rate on adenine-free plates so that their frequency on day 16 after plating was 1.5 × 10(-4 )for wild-type and 1.0 × 10(-2 )for the Exo(- )strain. In the Exo(- )strain, all revertants arising under adenine starvation are suppressors of the original mutation, most possessed additional nutritional requirements, and 50% of them were temperature sensitive. CONCLUSIONS: Adenine starvation is highly mutagenic in yeast. The deficiency in the polymerase delta proofreading activity in strains with the pol3-01 mutation leads to a further 66-fold increase of the rate of mutations. Our data suggest that adenine starvation induces genome-wide hyper-mutagenesis in the Exo(- )strain.
format Text
id pubmed-544876
institution National Center for Biotechnology Information
language English
publishDate 2004
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-5448762005-01-21 The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine Achilli, Alessandro Matmati, Nabil Casalone, Enrico Morpurgo, Giorgio Lucaccioni, Angela Pavlov, Youri I Babudri, Nora BMC Genet Research Article BACKGROUND: Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-documented phenomenon of an unknown mechanism. We have previously shown that the polymerase delta proofreading activity controls spontaneous mutagenesis in cells starved for histidine. To obtain further information, we compared the effect of adenine starvation on mutagenesis in wild-type cells and, in cells lacking the proofreading activity of polymerase delta (phenotype Exo(-), mutation pol3-01). RESULTS: Ade(+ )revertants accumulated at a very high rate on adenine-free plates so that their frequency on day 16 after plating was 1.5 × 10(-4 )for wild-type and 1.0 × 10(-2 )for the Exo(- )strain. In the Exo(- )strain, all revertants arising under adenine starvation are suppressors of the original mutation, most possessed additional nutritional requirements, and 50% of them were temperature sensitive. CONCLUSIONS: Adenine starvation is highly mutagenic in yeast. The deficiency in the polymerase delta proofreading activity in strains with the pol3-01 mutation leads to a further 66-fold increase of the rate of mutations. Our data suggest that adenine starvation induces genome-wide hyper-mutagenesis in the Exo(- )strain. BioMed Central 2004-12-23 /pmc/articles/PMC544876/ /pubmed/15617571 http://dx.doi.org/10.1186/1471-2156-5-34 Text en Copyright © 2004 Achilli et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Achilli, Alessandro
Matmati, Nabil
Casalone, Enrico
Morpurgo, Giorgio
Lucaccioni, Angela
Pavlov, Youri I
Babudri, Nora
The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine
title The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine
title_full The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine
title_fullStr The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine
title_full_unstemmed The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine
title_short The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine
title_sort exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient saccharomyces cerevisiae strain starved for adenine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544876/
https://www.ncbi.nlm.nih.gov/pubmed/15617571
http://dx.doi.org/10.1186/1471-2156-5-34
work_keys_str_mv AT achillialessandro theexceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT matmatinabil theexceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT casaloneenrico theexceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT morpurgogiorgio theexceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT lucaccioniangela theexceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT pavlovyourii theexceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT babudrinora theexceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT achillialessandro exceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT matmatinabil exceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT casaloneenrico exceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT morpurgogiorgio exceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT lucaccioniangela exceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT pavlovyourii exceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine
AT babudrinora exceptionallyhighrateofspontaneousmutationsinthepolymerasedeltaproofreadingexonucleasedeficientsaccharomycescerevisiaestrainstarvedforadenine