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Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study

Models for the organization of sterols into regular arrays within phospholipid bilayers have been proposed previously. The existence of such arrays in real systems has been supported by the fact that concentration-dependent sterol properties show discontinuities at the cholesterol mole fractions cor...

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Autores principales: Melzak, Kathryn A., Melzak, Shirley A., Gizeli, Electra, Toca-Herrera, José L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448994/
http://dx.doi.org/10.3390/ma5112306
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author Melzak, Kathryn A.
Melzak, Shirley A.
Gizeli, Electra
Toca-Herrera, José L.
author_facet Melzak, Kathryn A.
Melzak, Shirley A.
Gizeli, Electra
Toca-Herrera, José L.
author_sort Melzak, Kathryn A.
collection PubMed
description Models for the organization of sterols into regular arrays within phospholipid bilayers have been proposed previously. The existence of such arrays in real systems has been supported by the fact that concentration-dependent sterol properties show discontinuities at the cholesterol mole fractions corresponding to regular lattice arrangements. Experimental results presented here are based on a surface plasmon resonance assay that was used to analyze rates of cyclodextrin-mediated removal of cholesterol from adsorbed liposomes at cholesterol mole fractions up to χ(C) = 0.55. Two kinetic pools of cholesterol were detected; there was a fast pool present at χ(C) > 0.25, and a slow pool, with a removal rate that was dependent on the initial χ(C) but that did not vary as χ(C) decreased during the course of one experiment. The cholesterol activity therefore seems to be affected by sample history as well as local concentration, which could be explained in terms of the formation of superlattices that are stable for relatively long times. We also describe a variation on the traditional lattice models, with phosphatidylcholine (PC) being treated as an arrangement of hexagonal tiles; the cholesterol is then introduced at any vertex point, without increasing the total area occupied by all the lipid molecules. This model is consistent with Langmuir trough measurements of total lipid area and provides a simple explanation for the maximum solubility of cholesterol in the PC bilayer.
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spelling pubmed-54489942017-07-28 Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study Melzak, Kathryn A. Melzak, Shirley A. Gizeli, Electra Toca-Herrera, José L. Materials (Basel) Article Models for the organization of sterols into regular arrays within phospholipid bilayers have been proposed previously. The existence of such arrays in real systems has been supported by the fact that concentration-dependent sterol properties show discontinuities at the cholesterol mole fractions corresponding to regular lattice arrangements. Experimental results presented here are based on a surface plasmon resonance assay that was used to analyze rates of cyclodextrin-mediated removal of cholesterol from adsorbed liposomes at cholesterol mole fractions up to χ(C) = 0.55. Two kinetic pools of cholesterol were detected; there was a fast pool present at χ(C) > 0.25, and a slow pool, with a removal rate that was dependent on the initial χ(C) but that did not vary as χ(C) decreased during the course of one experiment. The cholesterol activity therefore seems to be affected by sample history as well as local concentration, which could be explained in terms of the formation of superlattices that are stable for relatively long times. We also describe a variation on the traditional lattice models, with phosphatidylcholine (PC) being treated as an arrangement of hexagonal tiles; the cholesterol is then introduced at any vertex point, without increasing the total area occupied by all the lipid molecules. This model is consistent with Langmuir trough measurements of total lipid area and provides a simple explanation for the maximum solubility of cholesterol in the PC bilayer. MDPI 2012-11-13 /pmc/articles/PMC5448994/ http://dx.doi.org/10.3390/ma5112306 Text en © 2012 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Melzak, Kathryn A.
Melzak, Shirley A.
Gizeli, Electra
Toca-Herrera, José L.
Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study
title Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study
title_full Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study
title_fullStr Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study
title_full_unstemmed Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study
title_short Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study
title_sort cholesterol organization in phosphatidylcholine liposomes: a surface plasmon resonance study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448994/
http://dx.doi.org/10.3390/ma5112306
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