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Pulmonary Inflammation of Well-Dispersed Multi-Wall Carbon Nanotubes Following Intratracheal Instillation: Toxicity by Fiber of 1–5 µm in Length
The pulmonary toxicity of multi-wall carbon nanotubes (MWCNT) were examined by intratracheal instillation. We prepared a well-dispersed MWCNT dispersion including MWCNTs of 3.71 µm geometric average length. The fiber length of most of the MWCNTs in the dispersion was 10 µm or less. The MWCNT dispers...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449071/ http://dx.doi.org/10.3390/ma5122833 |
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author | Horie, Masanori Stowe, Mayumi Kambara, Tatsunori Lee, Byeong Woo Endoh, Shigehisa Maru, Junko Oyabu, Takako Myojo, Toshihiko Ogami, Akira Uchida, Kunio Yamamoto, Kazuhiro Kobayashi, Norihiro Kuroda, Estushi Nakazato, Tetsuya Morimoto, Yasuo |
author_facet | Horie, Masanori Stowe, Mayumi Kambara, Tatsunori Lee, Byeong Woo Endoh, Shigehisa Maru, Junko Oyabu, Takako Myojo, Toshihiko Ogami, Akira Uchida, Kunio Yamamoto, Kazuhiro Kobayashi, Norihiro Kuroda, Estushi Nakazato, Tetsuya Morimoto, Yasuo |
author_sort | Horie, Masanori |
collection | PubMed |
description | The pulmonary toxicity of multi-wall carbon nanotubes (MWCNT) were examined by intratracheal instillation. We prepared a well-dispersed MWCNT dispersion including MWCNTs of 3.71 µm geometric average length. The fiber length of most of the MWCNTs in the dispersion was 10 µm or less. The MWCNT dispersion was administered to rat lung by single intratracheal instillation at doses of 0.2 mg and 0.6 mg/rat. Bronchoalveolar lavage fluid (BALF) was collected at 3 days, 1 week, 1 month, 3 months, and 6 months after instillation. The influences of the longer MWCNTs on the induction of inflammation and oxidative stress were examined by the number of neutrophils, cytokine induced neutrophil chemoattractant-1 (CINC-1), CINC-2, CINC-3 and HO-1 in the BALF. Additionally, ho-1 gene expression in the lung was examined. The intratracheal instillation of MWCNT induced transient inflammation dose dependently in the lung. The number of neutrophils was highest at 3 days after instillation and then decreased. However, the neutrophils in the MWCNT administered animals tended to be higher than in the control group until 3 months after instillation. The CINC-1 and CINC-2 concentrations in the BALF increased at 1 month after instillation. There were no significant differences in CINC-3 and HO-1 between the MWCNT administered animals and the control animals. These results revealed that the MWCNTs of 1–10 µm in length induced persistent inflammation in rat lung. There were no remarkable differences between the MWCNTs in the present study and previously reported, shorter MWCNTs prepared from “the same” raw MWCNT material. |
format | Online Article Text |
id | pubmed-5449071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54490712017-07-28 Pulmonary Inflammation of Well-Dispersed Multi-Wall Carbon Nanotubes Following Intratracheal Instillation: Toxicity by Fiber of 1–5 µm in Length Horie, Masanori Stowe, Mayumi Kambara, Tatsunori Lee, Byeong Woo Endoh, Shigehisa Maru, Junko Oyabu, Takako Myojo, Toshihiko Ogami, Akira Uchida, Kunio Yamamoto, Kazuhiro Kobayashi, Norihiro Kuroda, Estushi Nakazato, Tetsuya Morimoto, Yasuo Materials (Basel) Article The pulmonary toxicity of multi-wall carbon nanotubes (MWCNT) were examined by intratracheal instillation. We prepared a well-dispersed MWCNT dispersion including MWCNTs of 3.71 µm geometric average length. The fiber length of most of the MWCNTs in the dispersion was 10 µm or less. The MWCNT dispersion was administered to rat lung by single intratracheal instillation at doses of 0.2 mg and 0.6 mg/rat. Bronchoalveolar lavage fluid (BALF) was collected at 3 days, 1 week, 1 month, 3 months, and 6 months after instillation. The influences of the longer MWCNTs on the induction of inflammation and oxidative stress were examined by the number of neutrophils, cytokine induced neutrophil chemoattractant-1 (CINC-1), CINC-2, CINC-3 and HO-1 in the BALF. Additionally, ho-1 gene expression in the lung was examined. The intratracheal instillation of MWCNT induced transient inflammation dose dependently in the lung. The number of neutrophils was highest at 3 days after instillation and then decreased. However, the neutrophils in the MWCNT administered animals tended to be higher than in the control group until 3 months after instillation. The CINC-1 and CINC-2 concentrations in the BALF increased at 1 month after instillation. There were no significant differences in CINC-3 and HO-1 between the MWCNT administered animals and the control animals. These results revealed that the MWCNTs of 1–10 µm in length induced persistent inflammation in rat lung. There were no remarkable differences between the MWCNTs in the present study and previously reported, shorter MWCNTs prepared from “the same” raw MWCNT material. MDPI 2012-12-13 /pmc/articles/PMC5449071/ http://dx.doi.org/10.3390/ma5122833 Text en © 2012 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Horie, Masanori Stowe, Mayumi Kambara, Tatsunori Lee, Byeong Woo Endoh, Shigehisa Maru, Junko Oyabu, Takako Myojo, Toshihiko Ogami, Akira Uchida, Kunio Yamamoto, Kazuhiro Kobayashi, Norihiro Kuroda, Estushi Nakazato, Tetsuya Morimoto, Yasuo Pulmonary Inflammation of Well-Dispersed Multi-Wall Carbon Nanotubes Following Intratracheal Instillation: Toxicity by Fiber of 1–5 µm in Length |
title | Pulmonary Inflammation of Well-Dispersed Multi-Wall Carbon Nanotubes Following Intratracheal Instillation: Toxicity by Fiber of 1–5 µm in Length |
title_full | Pulmonary Inflammation of Well-Dispersed Multi-Wall Carbon Nanotubes Following Intratracheal Instillation: Toxicity by Fiber of 1–5 µm in Length |
title_fullStr | Pulmonary Inflammation of Well-Dispersed Multi-Wall Carbon Nanotubes Following Intratracheal Instillation: Toxicity by Fiber of 1–5 µm in Length |
title_full_unstemmed | Pulmonary Inflammation of Well-Dispersed Multi-Wall Carbon Nanotubes Following Intratracheal Instillation: Toxicity by Fiber of 1–5 µm in Length |
title_short | Pulmonary Inflammation of Well-Dispersed Multi-Wall Carbon Nanotubes Following Intratracheal Instillation: Toxicity by Fiber of 1–5 µm in Length |
title_sort | pulmonary inflammation of well-dispersed multi-wall carbon nanotubes following intratracheal instillation: toxicity by fiber of 1–5 µm in length |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449071/ http://dx.doi.org/10.3390/ma5122833 |
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