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A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis
The RecBCD complex is a key factor in DNA metabolism. This protein complex harbors a processive nuclease and two helicases activities that give it the ability to process duplex DNA ends. These enzymatic activities make RecBCD a major player in double strand break repair, conjugational recombination...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449595/ https://www.ncbi.nlm.nih.gov/pubmed/28369478 http://dx.doi.org/10.1093/nar/gkx217 |
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author | Laureti, Luisa Lee, Lara Philippin, Gaëlle Pagès, Vincent |
author_facet | Laureti, Luisa Lee, Lara Philippin, Gaëlle Pagès, Vincent |
author_sort | Laureti, Luisa |
collection | PubMed |
description | The RecBCD complex is a key factor in DNA metabolism. This protein complex harbors a processive nuclease and two helicases activities that give it the ability to process duplex DNA ends. These enzymatic activities make RecBCD a major player in double strand break repair, conjugational recombination and degradation of linear DNA. In this work, we unravel a new role of the RecBCD complex in the processing of DNA single-strand gaps that are generated at DNA replication-blocking lesions. We show that independently of its nuclease or helicase activities, the entire RecBCD complex is required for recombinational repair of the gap and efficient translesion synthesis. Since none of the catalytic functions of RecBCD are required for those processes, we surmise that the complex acts as a structural element that stabilizes the blocked replication fork, allowing efficient DNA damage tolerance. |
format | Online Article Text |
id | pubmed-5449595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54495952017-06-05 A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis Laureti, Luisa Lee, Lara Philippin, Gaëlle Pagès, Vincent Nucleic Acids Res Genome Integrity, Repair and Replication The RecBCD complex is a key factor in DNA metabolism. This protein complex harbors a processive nuclease and two helicases activities that give it the ability to process duplex DNA ends. These enzymatic activities make RecBCD a major player in double strand break repair, conjugational recombination and degradation of linear DNA. In this work, we unravel a new role of the RecBCD complex in the processing of DNA single-strand gaps that are generated at DNA replication-blocking lesions. We show that independently of its nuclease or helicase activities, the entire RecBCD complex is required for recombinational repair of the gap and efficient translesion synthesis. Since none of the catalytic functions of RecBCD are required for those processes, we surmise that the complex acts as a structural element that stabilizes the blocked replication fork, allowing efficient DNA damage tolerance. Oxford University Press 2017-06-02 2017-03-29 /pmc/articles/PMC5449595/ /pubmed/28369478 http://dx.doi.org/10.1093/nar/gkx217 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Genome Integrity, Repair and Replication Laureti, Luisa Lee, Lara Philippin, Gaëlle Pagès, Vincent A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis |
title | A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis |
title_full | A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis |
title_fullStr | A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis |
title_full_unstemmed | A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis |
title_short | A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis |
title_sort | non-catalytic role of recbcd in homology directed gap repair and translesion synthesis |
topic | Genome Integrity, Repair and Replication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449595/ https://www.ncbi.nlm.nih.gov/pubmed/28369478 http://dx.doi.org/10.1093/nar/gkx217 |
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