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Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection

Delivery of DNA to cells and its subsequent integration into the host genome is a fundamental task in molecular biology, biotechnology and gene therapy. Here we describe an IP-free one-step method that enables stable genome integration into either prokaryotic or eukaryotic cells. A synthetic mariner...

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Detalles Bibliográficos
Autores principales:	Trubitsyna, Maryia, Michlewski, Gracjan, Finnegan, David J., Elfick, Alistair, Rosser, Susan J., Richardson, Julia M., French, Christopher E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449632/ https://www.ncbi.nlm.nih.gov/pubmed/28204586 http://dx.doi.org/10.1093/nar/gkx113

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