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In Vivo Imaging of Influenza Virus Infection in Immunized Mice
Immunization is the cornerstone of seasonal influenza control and represents an important component of pandemic preparedness strategies. Using a bioluminescent reporter virus, we demonstrate the application of noninvasive in vivo imaging system (IVIS) technology to evaluate the preclinical efficacy...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449660/ https://www.ncbi.nlm.nih.gov/pubmed/28559489 http://dx.doi.org/10.1128/mBio.00714-17 |
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author | Czakó, Rita Vogel, Leatrice Lamirande, Elaine W. Bock, Kevin W. Moore, Ian N. Ellebedy, Ali H. Ahmed, Rafi Mehle, Andrew Subbarao, Kanta |
author_facet | Czakó, Rita Vogel, Leatrice Lamirande, Elaine W. Bock, Kevin W. Moore, Ian N. Ellebedy, Ali H. Ahmed, Rafi Mehle, Andrew Subbarao, Kanta |
author_sort | Czakó, Rita |
collection | PubMed |
description | Immunization is the cornerstone of seasonal influenza control and represents an important component of pandemic preparedness strategies. Using a bioluminescent reporter virus, we demonstrate the application of noninvasive in vivo imaging system (IVIS) technology to evaluate the preclinical efficacy of candidate vaccines and immunotherapy in a mouse model of influenza. Sequential imaging revealed distinct spatiotemporal kinetics of bioluminescence in groups of mice passively or actively immunized by various strategies that accelerated the clearance of the challenge virus at different rates and by distinct mechanisms. Imaging findings were consistent with conclusions derived from virus titers in the lungs and, notably, were more informative than conventional efficacy endpoints in some cases. Our findings demonstrate the reliability of IVIS as a qualitative approach to support preclinical evaluation of candidate medical countermeasures for influenza in mice. |
format | Online Article Text |
id | pubmed-5449660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-54496602017-06-01 In Vivo Imaging of Influenza Virus Infection in Immunized Mice Czakó, Rita Vogel, Leatrice Lamirande, Elaine W. Bock, Kevin W. Moore, Ian N. Ellebedy, Ali H. Ahmed, Rafi Mehle, Andrew Subbarao, Kanta mBio Research Article Immunization is the cornerstone of seasonal influenza control and represents an important component of pandemic preparedness strategies. Using a bioluminescent reporter virus, we demonstrate the application of noninvasive in vivo imaging system (IVIS) technology to evaluate the preclinical efficacy of candidate vaccines and immunotherapy in a mouse model of influenza. Sequential imaging revealed distinct spatiotemporal kinetics of bioluminescence in groups of mice passively or actively immunized by various strategies that accelerated the clearance of the challenge virus at different rates and by distinct mechanisms. Imaging findings were consistent with conclusions derived from virus titers in the lungs and, notably, were more informative than conventional efficacy endpoints in some cases. Our findings demonstrate the reliability of IVIS as a qualitative approach to support preclinical evaluation of candidate medical countermeasures for influenza in mice. American Society for Microbiology 2017-05-30 /pmc/articles/PMC5449660/ /pubmed/28559489 http://dx.doi.org/10.1128/mBio.00714-17 Text en Copyright © 2017 Czakó et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Czakó, Rita Vogel, Leatrice Lamirande, Elaine W. Bock, Kevin W. Moore, Ian N. Ellebedy, Ali H. Ahmed, Rafi Mehle, Andrew Subbarao, Kanta In Vivo Imaging of Influenza Virus Infection in Immunized Mice |
title | In Vivo Imaging of Influenza Virus Infection in Immunized Mice |
title_full | In Vivo Imaging of Influenza Virus Infection in Immunized Mice |
title_fullStr | In Vivo Imaging of Influenza Virus Infection in Immunized Mice |
title_full_unstemmed | In Vivo Imaging of Influenza Virus Infection in Immunized Mice |
title_short | In Vivo Imaging of Influenza Virus Infection in Immunized Mice |
title_sort | in vivo imaging of influenza virus infection in immunized mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449660/ https://www.ncbi.nlm.nih.gov/pubmed/28559489 http://dx.doi.org/10.1128/mBio.00714-17 |
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