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Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts

Piper betle (PB) is a traditional medicine that is widely used to treat different diseases around Asian region. The leaf extracts contain various bioactive compounds, which were reported to have antidiabetic, antibacterial, anti-inflammatory, antioxidant, and anticancer effects. In this study, the e...

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Autores principales: Durani, Lina Wati, Khor, Shy Cian, Tan, Jen Kit, Chua, Kien Hui, Mohd Yusof, Yasmin Anum, Makpol, Suzana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449738/
https://www.ncbi.nlm.nih.gov/pubmed/28596968
http://dx.doi.org/10.1155/2017/6894026
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author Durani, Lina Wati
Khor, Shy Cian
Tan, Jen Kit
Chua, Kien Hui
Mohd Yusof, Yasmin Anum
Makpol, Suzana
author_facet Durani, Lina Wati
Khor, Shy Cian
Tan, Jen Kit
Chua, Kien Hui
Mohd Yusof, Yasmin Anum
Makpol, Suzana
author_sort Durani, Lina Wati
collection PubMed
description Piper betle (PB) is a traditional medicine that is widely used to treat different diseases around Asian region. The leaf extracts contain various bioactive compounds, which were reported to have antidiabetic, antibacterial, anti-inflammatory, antioxidant, and anticancer effects. In this study, the effect of PB aqueous extracts on replicative senescent human diploid fibroblasts (HDFs) was investigated by determining the expressions of senescence-associated genes using quantitative PCR. Our results showed that PB extracts at 0.4 mg/ml can improve cell proliferation of young (143%), presenescent (127.3%), and senescent (157.3%) HDFs. Increased expressions of PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were observed in senescent HDFs compared to young and/or presenescent HDFs. Treatment with PB extracts modulates the transcriptional profile changes in senescent HDFs. By contrast, expressions of SOD1 increased, whereas GPX1, PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were decreased in PB-treated senescent HDFs compared to untreated senescent HDFs. In conclusion, this study indicates the modulation of PB extracts on senescence-associated genes expression of replicative senescent HDFs. Further studies warrant determining the mechanism of PB in modulating replicative senescence of HDFs through these signaling pathways.
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spelling pubmed-54497382017-06-08 Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts Durani, Lina Wati Khor, Shy Cian Tan, Jen Kit Chua, Kien Hui Mohd Yusof, Yasmin Anum Makpol, Suzana Biomed Res Int Research Article Piper betle (PB) is a traditional medicine that is widely used to treat different diseases around Asian region. The leaf extracts contain various bioactive compounds, which were reported to have antidiabetic, antibacterial, anti-inflammatory, antioxidant, and anticancer effects. In this study, the effect of PB aqueous extracts on replicative senescent human diploid fibroblasts (HDFs) was investigated by determining the expressions of senescence-associated genes using quantitative PCR. Our results showed that PB extracts at 0.4 mg/ml can improve cell proliferation of young (143%), presenescent (127.3%), and senescent (157.3%) HDFs. Increased expressions of PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were observed in senescent HDFs compared to young and/or presenescent HDFs. Treatment with PB extracts modulates the transcriptional profile changes in senescent HDFs. By contrast, expressions of SOD1 increased, whereas GPX1, PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were decreased in PB-treated senescent HDFs compared to untreated senescent HDFs. In conclusion, this study indicates the modulation of PB extracts on senescence-associated genes expression of replicative senescent HDFs. Further studies warrant determining the mechanism of PB in modulating replicative senescence of HDFs through these signaling pathways. Hindawi 2017 2017-05-17 /pmc/articles/PMC5449738/ /pubmed/28596968 http://dx.doi.org/10.1155/2017/6894026 Text en Copyright © 2017 Lina Wati Durani et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Durani, Lina Wati
Khor, Shy Cian
Tan, Jen Kit
Chua, Kien Hui
Mohd Yusof, Yasmin Anum
Makpol, Suzana
Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts
title Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts
title_full Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts
title_fullStr Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts
title_full_unstemmed Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts
title_short Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts
title_sort piper betle l. modulates senescence-associated genes expression in replicative senescent human diploid fibroblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449738/
https://www.ncbi.nlm.nih.gov/pubmed/28596968
http://dx.doi.org/10.1155/2017/6894026
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