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Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae

Alcoholic liver disease (ALD) is a series of abnormalities of liver function, including alcoholic steatosis, steatohepatitis, and cirrhosis. Hesperidin, the major constituent of flavanone in grapefruit, is proved to play a role in antioxidation, anti-inflammation, and reducing multiple organs damage...

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Autores principales: Zhou, Zhenting, Zhong, Weichao, Lin, Haiyan, Huang, Peng, Ma, Ning, Zhang, Yuqing, Zhou, Chuying, Lai, Yuling, Huang, Shaohui, Huang, Shiying, Gao, Lei, Lv, Zhiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449749/
https://www.ncbi.nlm.nih.gov/pubmed/28596796
http://dx.doi.org/10.1155/2017/7282653
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author Zhou, Zhenting
Zhong, Weichao
Lin, Haiyan
Huang, Peng
Ma, Ning
Zhang, Yuqing
Zhou, Chuying
Lai, Yuling
Huang, Shaohui
Huang, Shiying
Gao, Lei
Lv, Zhiping
author_facet Zhou, Zhenting
Zhong, Weichao
Lin, Haiyan
Huang, Peng
Ma, Ning
Zhang, Yuqing
Zhou, Chuying
Lai, Yuling
Huang, Shaohui
Huang, Shiying
Gao, Lei
Lv, Zhiping
author_sort Zhou, Zhenting
collection PubMed
description Alcoholic liver disease (ALD) is a series of abnormalities of liver function, including alcoholic steatosis, steatohepatitis, and cirrhosis. Hesperidin, the major constituent of flavanone in grapefruit, is proved to play a role in antioxidation, anti-inflammation, and reducing multiple organs damage in various animal experiments. However, the underlying mechanism of resistance to alcoholic liver injury is still unclear. Thus, we aimed to investigate the protective effects of hesperidin against ALD and its molecular mechanism in this study. We established an ALD zebrafish larvae model induced by 350 mM ethanol for 32 hours, using wild-type and transgenic line with liver-specific eGFP expression Tg (lfabp10α:eGFP) zebrafish larvae (4 dpf). The results revealed that hesperidin dramatically reduced the hepatic morphological damage and the expressions of alcohol and lipid metabolism related genes, including cyp2y3, cyp3a65, hmgcra, hmgcrb, fasn, and fads2 compared with ALD model. Moreover, the findings demonstrated that hesperidin alleviated hepatic damage as well, which is reflected by the expressions of endoplasmic reticulum stress and DNA damage related genes (chop, gadd45αa, and edem1). In conclusion, this study revealed that hesperidin can inhibit alcoholic damage to liver of zebrafish larvae by reducing endoplasmic reticulum stress and DNA damage, regulating alcohol and lipid metabolism.
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spelling pubmed-54497492017-06-08 Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae Zhou, Zhenting Zhong, Weichao Lin, Haiyan Huang, Peng Ma, Ning Zhang, Yuqing Zhou, Chuying Lai, Yuling Huang, Shaohui Huang, Shiying Gao, Lei Lv, Zhiping Evid Based Complement Alternat Med Research Article Alcoholic liver disease (ALD) is a series of abnormalities of liver function, including alcoholic steatosis, steatohepatitis, and cirrhosis. Hesperidin, the major constituent of flavanone in grapefruit, is proved to play a role in antioxidation, anti-inflammation, and reducing multiple organs damage in various animal experiments. However, the underlying mechanism of resistance to alcoholic liver injury is still unclear. Thus, we aimed to investigate the protective effects of hesperidin against ALD and its molecular mechanism in this study. We established an ALD zebrafish larvae model induced by 350 mM ethanol for 32 hours, using wild-type and transgenic line with liver-specific eGFP expression Tg (lfabp10α:eGFP) zebrafish larvae (4 dpf). The results revealed that hesperidin dramatically reduced the hepatic morphological damage and the expressions of alcohol and lipid metabolism related genes, including cyp2y3, cyp3a65, hmgcra, hmgcrb, fasn, and fads2 compared with ALD model. Moreover, the findings demonstrated that hesperidin alleviated hepatic damage as well, which is reflected by the expressions of endoplasmic reticulum stress and DNA damage related genes (chop, gadd45αa, and edem1). In conclusion, this study revealed that hesperidin can inhibit alcoholic damage to liver of zebrafish larvae by reducing endoplasmic reticulum stress and DNA damage, regulating alcohol and lipid metabolism. Hindawi 2017 2017-05-17 /pmc/articles/PMC5449749/ /pubmed/28596796 http://dx.doi.org/10.1155/2017/7282653 Text en Copyright © 2017 Zhenting Zhou et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhou, Zhenting
Zhong, Weichao
Lin, Haiyan
Huang, Peng
Ma, Ning
Zhang, Yuqing
Zhou, Chuying
Lai, Yuling
Huang, Shaohui
Huang, Shiying
Gao, Lei
Lv, Zhiping
Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae
title Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae
title_full Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae
title_fullStr Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae
title_full_unstemmed Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae
title_short Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae
title_sort hesperidin protects against acute alcoholic injury through improving lipid metabolism and cell damage in zebrafish larvae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449749/
https://www.ncbi.nlm.nih.gov/pubmed/28596796
http://dx.doi.org/10.1155/2017/7282653
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