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Evaluating the accuracy of amplicon-based microbiome computational pipelines on simulated human gut microbial communities

BACKGROUND: Microbiome studies commonly use 16S rRNA gene amplicon sequencing to characterize microbial communities. Errors introduced at multiple steps in this process can affect the interpretation of the data. Here we evaluate the accuracy of operational taxonomic unit (OTU) generation, taxonomic...

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Detalles Bibliográficos
Autores principales:	Golob, Jonathan L., Margolis, Elisa, Hoffman, Noah G., Fredricks, David N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450146/ https://www.ncbi.nlm.nih.gov/pubmed/28558684 http://dx.doi.org/10.1186/s12859-017-1690-0

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