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Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena

The nuclear pore complex (NPC), a gateway for nucleocytoplasmic trafficking, is composed of ∼30 different proteins called nucleoporins. It remains unknown whether the NPCs within a species are homogeneous or vary depending on the cell type or physiological condition. Here, we present evidence for co...

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Autores principales: Iwamoto, Masaaki, Osakada, Hiroko, Mori, Chie, Fukuda, Yasuhiro, Nagao, Koji, Obuse, Chikashi, Hiraoka, Yasushi, Haraguchi, Tokuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450191/
https://www.ncbi.nlm.nih.gov/pubmed/28386019
http://dx.doi.org/10.1242/jcs.199398
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author Iwamoto, Masaaki
Osakada, Hiroko
Mori, Chie
Fukuda, Yasuhiro
Nagao, Koji
Obuse, Chikashi
Hiraoka, Yasushi
Haraguchi, Tokuko
author_facet Iwamoto, Masaaki
Osakada, Hiroko
Mori, Chie
Fukuda, Yasuhiro
Nagao, Koji
Obuse, Chikashi
Hiraoka, Yasushi
Haraguchi, Tokuko
author_sort Iwamoto, Masaaki
collection PubMed
description The nuclear pore complex (NPC), a gateway for nucleocytoplasmic trafficking, is composed of ∼30 different proteins called nucleoporins. It remains unknown whether the NPCs within a species are homogeneous or vary depending on the cell type or physiological condition. Here, we present evidence for compositionally distinct NPCs that form within a single cell in a binucleated ciliate. In Tetrahymena thermophila, each cell contains both a transcriptionally active macronucleus (MAC) and a germline micronucleus (MIC). By combining in silico analysis, mass spectrometry analysis for immuno-isolated proteins and subcellular localization analysis of GFP-fused proteins, we identified numerous novel components of MAC and MIC NPCs. Core members of the Nup107–Nup160 scaffold complex were enriched in MIC NPCs. Strikingly, two paralogs of Nup214 and of Nup153 localized exclusively to either the MAC or MIC NPCs. Furthermore, the transmembrane components Pom121 and Pom82 localize exclusively to MAC and MIC NPCs, respectively. Our results argue that functional nuclear dimorphism in ciliates is likely to depend on the compositional and structural specificity of NPCs.
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spelling pubmed-54501912017-06-13 Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena Iwamoto, Masaaki Osakada, Hiroko Mori, Chie Fukuda, Yasuhiro Nagao, Koji Obuse, Chikashi Hiraoka, Yasushi Haraguchi, Tokuko J Cell Sci Research Article The nuclear pore complex (NPC), a gateway for nucleocytoplasmic trafficking, is composed of ∼30 different proteins called nucleoporins. It remains unknown whether the NPCs within a species are homogeneous or vary depending on the cell type or physiological condition. Here, we present evidence for compositionally distinct NPCs that form within a single cell in a binucleated ciliate. In Tetrahymena thermophila, each cell contains both a transcriptionally active macronucleus (MAC) and a germline micronucleus (MIC). By combining in silico analysis, mass spectrometry analysis for immuno-isolated proteins and subcellular localization analysis of GFP-fused proteins, we identified numerous novel components of MAC and MIC NPCs. Core members of the Nup107–Nup160 scaffold complex were enriched in MIC NPCs. Strikingly, two paralogs of Nup214 and of Nup153 localized exclusively to either the MAC or MIC NPCs. Furthermore, the transmembrane components Pom121 and Pom82 localize exclusively to MAC and MIC NPCs, respectively. Our results argue that functional nuclear dimorphism in ciliates is likely to depend on the compositional and structural specificity of NPCs. The Company of Biologists Ltd 2017-05-15 /pmc/articles/PMC5450191/ /pubmed/28386019 http://dx.doi.org/10.1242/jcs.199398 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Iwamoto, Masaaki
Osakada, Hiroko
Mori, Chie
Fukuda, Yasuhiro
Nagao, Koji
Obuse, Chikashi
Hiraoka, Yasushi
Haraguchi, Tokuko
Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena
title Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena
title_full Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena
title_fullStr Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena
title_full_unstemmed Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena
title_short Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena
title_sort compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate tetrahymena
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450191/
https://www.ncbi.nlm.nih.gov/pubmed/28386019
http://dx.doi.org/10.1242/jcs.199398
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