Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer

BACKGROUND: The association between Epstein-Barr virus (EBV) and the development of head and neck cancer was reported by many researchers. The aim of the present study was to detect EBV DNA and EBV antibodies in 110 Polish patients with oropharyngeal and laryngeal cancer compared to 40 healthy indiv...

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Autores principales: Fołtyn, Sylwia, Strycharz-Dudziak, Małgorzata, Drop, Bartłomiej, Boguszewska, Anastazja, Polz-Dacewicz, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450389/
https://www.ncbi.nlm.nih.gov/pubmed/28572837
http://dx.doi.org/10.1186/s13027-017-0141-x
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author Fołtyn, Sylwia
Strycharz-Dudziak, Małgorzata
Drop, Bartłomiej
Boguszewska, Anastazja
Polz-Dacewicz, Małgorzata
author_facet Fołtyn, Sylwia
Strycharz-Dudziak, Małgorzata
Drop, Bartłomiej
Boguszewska, Anastazja
Polz-Dacewicz, Małgorzata
author_sort Fołtyn, Sylwia
collection PubMed
description BACKGROUND: The association between Epstein-Barr virus (EBV) and the development of head and neck cancer was reported by many researchers. The aim of the present study was to detect EBV DNA and EBV antibodies in 110 Polish patients with oropharyngeal and laryngeal cancer compared to 40 healthy individuals. METHODS: Frozen tumor tissue fragments were tested using nested PCR assay for EBV DNA detection. Sera from all individuals were investigated using ELISA tests to detect the presence of VCA IgM and IgG, EBNA IgG, EA IgG. RESULTS: EBV DNA was detected in 52.7% of the patients (25% in controls). EBVCA were detected in 94.5%, EBNA in 96.4% and EA in 94.5% of patients. The significantly higher level of EA in the patients suggests EBV reactivation. The majority of patients (83%) were infected with wild-type EBV. CONCLUSION: Our study showed that this variant seems to be associated with oropharyngeal and laryngeal cancer in the Polish population.
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spelling pubmed-54503892017-06-01 Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer Fołtyn, Sylwia Strycharz-Dudziak, Małgorzata Drop, Bartłomiej Boguszewska, Anastazja Polz-Dacewicz, Małgorzata Infect Agent Cancer Research Article BACKGROUND: The association between Epstein-Barr virus (EBV) and the development of head and neck cancer was reported by many researchers. The aim of the present study was to detect EBV DNA and EBV antibodies in 110 Polish patients with oropharyngeal and laryngeal cancer compared to 40 healthy individuals. METHODS: Frozen tumor tissue fragments were tested using nested PCR assay for EBV DNA detection. Sera from all individuals were investigated using ELISA tests to detect the presence of VCA IgM and IgG, EBNA IgG, EA IgG. RESULTS: EBV DNA was detected in 52.7% of the patients (25% in controls). EBVCA were detected in 94.5%, EBNA in 96.4% and EA in 94.5% of patients. The significantly higher level of EA in the patients suggests EBV reactivation. The majority of patients (83%) were infected with wild-type EBV. CONCLUSION: Our study showed that this variant seems to be associated with oropharyngeal and laryngeal cancer in the Polish population. BioMed Central 2017-05-30 /pmc/articles/PMC5450389/ /pubmed/28572837 http://dx.doi.org/10.1186/s13027-017-0141-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Fołtyn, Sylwia
Strycharz-Dudziak, Małgorzata
Drop, Bartłomiej
Boguszewska, Anastazja
Polz-Dacewicz, Małgorzata
Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer
title Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer
title_full Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer
title_fullStr Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer
title_full_unstemmed Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer
title_short Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer
title_sort serum ebv antibodies and lmp-1 in polish patients with oropharyngeal and laryngeal cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450389/
https://www.ncbi.nlm.nih.gov/pubmed/28572837
http://dx.doi.org/10.1186/s13027-017-0141-x
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