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Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica

Sponges, despite their simple body plan, discriminate between self and nonself with remarkable specificity. Sponge grafting experiments simulate the effects of natural self or nonself contact under laboratory conditions. Here we take a transcriptomic approach to investigate the temporal response to...

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Detalles Bibliográficos
Autores principales: Grice, Laura F., Degnan, Bernard M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450542/
https://www.ncbi.nlm.nih.gov/pubmed/28492509
http://dx.doi.org/10.3390/md15050136
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author Grice, Laura F.
Degnan, Bernard M.
author_facet Grice, Laura F.
Degnan, Bernard M.
author_sort Grice, Laura F.
collection PubMed
description Sponges, despite their simple body plan, discriminate between self and nonself with remarkable specificity. Sponge grafting experiments simulate the effects of natural self or nonself contact under laboratory conditions. Here we take a transcriptomic approach to investigate the temporal response to self and nonself grafts in the marine demosponge Amphimedon queenslandica. Auto- and allografts were established, observed and sampled over a period of three days, over which time the grafts either rejected or accepted, depending on the identity of the paired individuals, in a replicable and predictable manner. Fourteen transcriptomes were generated that spanned the auto- and allograft responses. Self grafts fuse completely in under three days, and the process appears to be controlled by relatively few genes. In contrast, nonself grafting results in a complete lack of fusion after three days, and appears to involve a broad downregulation of normal biological processes, rather than the mounting of an intense defensive response.
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spelling pubmed-54505422017-06-05 Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica Grice, Laura F. Degnan, Bernard M. Mar Drugs Article Sponges, despite their simple body plan, discriminate between self and nonself with remarkable specificity. Sponge grafting experiments simulate the effects of natural self or nonself contact under laboratory conditions. Here we take a transcriptomic approach to investigate the temporal response to self and nonself grafts in the marine demosponge Amphimedon queenslandica. Auto- and allografts were established, observed and sampled over a period of three days, over which time the grafts either rejected or accepted, depending on the identity of the paired individuals, in a replicable and predictable manner. Fourteen transcriptomes were generated that spanned the auto- and allograft responses. Self grafts fuse completely in under three days, and the process appears to be controlled by relatively few genes. In contrast, nonself grafting results in a complete lack of fusion after three days, and appears to involve a broad downregulation of normal biological processes, rather than the mounting of an intense defensive response. MDPI 2017-05-11 /pmc/articles/PMC5450542/ /pubmed/28492509 http://dx.doi.org/10.3390/md15050136 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grice, Laura F.
Degnan, Bernard M.
Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica
title Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica
title_full Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica
title_fullStr Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica
title_full_unstemmed Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica
title_short Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica
title_sort transcriptomic profiling of the allorecognition response to grafting in the demosponge amphimedon queenslandica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450542/
https://www.ncbi.nlm.nih.gov/pubmed/28492509
http://dx.doi.org/10.3390/md15050136
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