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Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats

Testicular torsion/detorsion causes severe tissue damage due to ischemia/reperfusion injury. The present study investigated the protective effect of erythropoietin and sildenafil against ischemia/reperfusion injury following unilateral testicular torsion/detorsion in adult rats. A total of 28 adult...

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Autores principales: Kostakis, Ioannis D., Zavras, Nick, Damaskos, Christos, Sakellariou, Stratigoula, Korkolopoulou, Penelope, Misiakos, Evangelos P., Tsaparas, Petros, Vaos, George, Karatzas, Theodoros
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450555/
https://www.ncbi.nlm.nih.gov/pubmed/28587411
http://dx.doi.org/10.3892/etm.2017.4441
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author Kostakis, Ioannis D.
Zavras, Nick
Damaskos, Christos
Sakellariou, Stratigoula
Korkolopoulou, Penelope
Misiakos, Evangelos P.
Tsaparas, Petros
Vaos, George
Karatzas, Theodoros
author_facet Kostakis, Ioannis D.
Zavras, Nick
Damaskos, Christos
Sakellariou, Stratigoula
Korkolopoulou, Penelope
Misiakos, Evangelos P.
Tsaparas, Petros
Vaos, George
Karatzas, Theodoros
author_sort Kostakis, Ioannis D.
collection PubMed
description Testicular torsion/detorsion causes severe tissue damage due to ischemia/reperfusion injury. The present study investigated the protective effect of erythropoietin and sildenafil against ischemia/reperfusion injury following unilateral testicular torsion/detorsion in adult rats. A total of 28 adult male rats were included, and were divided into the following groups: Group A (n=5), sham operated; groups B (n=5), C (n=5), D (n=5) and E (n=8), undergoing right testis torsion and detorsion after 90 min. Group B received no drug treatment. Rats in the groups C and D received low-dose (1,000 IU/kg) or high-dose (3,000 IU/kg) erythropoietin, while those in group E received sildenafil (0.7 mg/kg), through intraperitoneal injection after 60 min of torsion. The right testis was extracted 24 h after detorsion, and the tissue was subjected to histopathological examination and immunohistochemical assessment of cleaved caspase-3 expression. Histological alterations and the quality of spermatogenesis were scored according to the Cosentino and the Johnsen scoring systems, respectively. The results demonstrated normal testicular architecture in group A, while the other groups showed ischemic cellular damages, with the worst scores observed in group B. Groups D and E presented better scores compared with group C. Regarding the quality of spermatogenesis, the best scores were observed in group A, and the worst in group B. Groups C, D and E presented similar results, which were improved in comparison to group B, however, not compared to group A. Furthermore, cleaved caspase-3 levels were lower in groups A, D and E, with equal results observed. Group C had higher levels of cleaved caspase-3 compared with these groups, but lower than group B, which presented the highest cleaved caspase-3 levels. In conclusion, erythropoietin and sildenafil protect testis from ischemia/reperfusion injury by decreasing cellular damage and attenuating apoptosis.
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spelling pubmed-54505552017-06-05 Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats Kostakis, Ioannis D. Zavras, Nick Damaskos, Christos Sakellariou, Stratigoula Korkolopoulou, Penelope Misiakos, Evangelos P. Tsaparas, Petros Vaos, George Karatzas, Theodoros Exp Ther Med Articles Testicular torsion/detorsion causes severe tissue damage due to ischemia/reperfusion injury. The present study investigated the protective effect of erythropoietin and sildenafil against ischemia/reperfusion injury following unilateral testicular torsion/detorsion in adult rats. A total of 28 adult male rats were included, and were divided into the following groups: Group A (n=5), sham operated; groups B (n=5), C (n=5), D (n=5) and E (n=8), undergoing right testis torsion and detorsion after 90 min. Group B received no drug treatment. Rats in the groups C and D received low-dose (1,000 IU/kg) or high-dose (3,000 IU/kg) erythropoietin, while those in group E received sildenafil (0.7 mg/kg), through intraperitoneal injection after 60 min of torsion. The right testis was extracted 24 h after detorsion, and the tissue was subjected to histopathological examination and immunohistochemical assessment of cleaved caspase-3 expression. Histological alterations and the quality of spermatogenesis were scored according to the Cosentino and the Johnsen scoring systems, respectively. The results demonstrated normal testicular architecture in group A, while the other groups showed ischemic cellular damages, with the worst scores observed in group B. Groups D and E presented better scores compared with group C. Regarding the quality of spermatogenesis, the best scores were observed in group A, and the worst in group B. Groups C, D and E presented similar results, which were improved in comparison to group B, however, not compared to group A. Furthermore, cleaved caspase-3 levels were lower in groups A, D and E, with equal results observed. Group C had higher levels of cleaved caspase-3 compared with these groups, but lower than group B, which presented the highest cleaved caspase-3 levels. In conclusion, erythropoietin and sildenafil protect testis from ischemia/reperfusion injury by decreasing cellular damage and attenuating apoptosis. D.A. Spandidos 2017-06 2017-05-08 /pmc/articles/PMC5450555/ /pubmed/28587411 http://dx.doi.org/10.3892/etm.2017.4441 Text en Copyright: © Kostakis et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Kostakis, Ioannis D.
Zavras, Nick
Damaskos, Christos
Sakellariou, Stratigoula
Korkolopoulou, Penelope
Misiakos, Evangelos P.
Tsaparas, Petros
Vaos, George
Karatzas, Theodoros
Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats
title Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats
title_full Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats
title_fullStr Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats
title_full_unstemmed Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats
title_short Erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats
title_sort erythropoietin and sildenafil protect against ischemia/reperfusion injury following testicular torsion in adult rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450555/
https://www.ncbi.nlm.nih.gov/pubmed/28587411
http://dx.doi.org/10.3892/etm.2017.4441
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