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Brain cell apoptosis inhibition by butylphthalide in Alzheimer's disease model in rats

The present study was designed to test the hypothesis that butylphthalide protects the brain of Alzheimer's disease (AD) model rats by inhibiting apoptosis. Ninety Sprague-Dawley rats were randomly divided into drug, control and blank groups of 30 rats in each. The rats in the drug and control...

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Autores principales: Song, Fu-Xia, Wang, Li, Liu, Hong, Wang, Ying-Li, Zou, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450572/
https://www.ncbi.nlm.nih.gov/pubmed/28587340
http://dx.doi.org/10.3892/etm.2017.4322
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author Song, Fu-Xia
Wang, Li
Liu, Hong
Wang, Ying-Li
Zou, Yong
author_facet Song, Fu-Xia
Wang, Li
Liu, Hong
Wang, Ying-Li
Zou, Yong
author_sort Song, Fu-Xia
collection PubMed
description The present study was designed to test the hypothesis that butylphthalide protects the brain of Alzheimer's disease (AD) model rats by inhibiting apoptosis. Ninety Sprague-Dawley rats were randomly divided into drug, control and blank groups of 30 rats in each. The rats in the drug and control groups were treated to induce AD. Then, the rats in the drug group were administered with butylphthalide daily, the rats in the AD control group were given normal saline, and the rats in the healthy group were fed routinely. All rats were sacrificed after 30 days; the brain tissues were used for testing for apoptosis by the terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling (TUNEL) staining method, for determining mitogen-activated protein kinase (MAPK), ERK and P21 protein by western blot analysis, and their cognate mRNA levels by RT-PCR. The results of the TUNEL staining indicated that apoptosis of the brain tissues of rats in the drug group was significantly less than that in the control group and blank group. The protein expression levels of MAPK in the drug group were significantly lower than that in the control group, but higher than that in the normal healthy group (P<0.05). The mRNA expression levels of MAPK in the drug group were significantly lower than those in the control group, but higher than those in the normal healthy group (P<0.05). Based on these results, butylphthalide showed a protective apoptosis-inhibition effect on the brain tissues of the AD rats and this seems to be a consequence of its inhibition of the expressions of MAPK mRNA and MAPK protein in the brain of the rat.
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spelling pubmed-54505722017-06-05 Brain cell apoptosis inhibition by butylphthalide in Alzheimer's disease model in rats Song, Fu-Xia Wang, Li Liu, Hong Wang, Ying-Li Zou, Yong Exp Ther Med Articles The present study was designed to test the hypothesis that butylphthalide protects the brain of Alzheimer's disease (AD) model rats by inhibiting apoptosis. Ninety Sprague-Dawley rats were randomly divided into drug, control and blank groups of 30 rats in each. The rats in the drug and control groups were treated to induce AD. Then, the rats in the drug group were administered with butylphthalide daily, the rats in the AD control group were given normal saline, and the rats in the healthy group were fed routinely. All rats were sacrificed after 30 days; the brain tissues were used for testing for apoptosis by the terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling (TUNEL) staining method, for determining mitogen-activated protein kinase (MAPK), ERK and P21 protein by western blot analysis, and their cognate mRNA levels by RT-PCR. The results of the TUNEL staining indicated that apoptosis of the brain tissues of rats in the drug group was significantly less than that in the control group and blank group. The protein expression levels of MAPK in the drug group were significantly lower than that in the control group, but higher than that in the normal healthy group (P<0.05). The mRNA expression levels of MAPK in the drug group were significantly lower than those in the control group, but higher than those in the normal healthy group (P<0.05). Based on these results, butylphthalide showed a protective apoptosis-inhibition effect on the brain tissues of the AD rats and this seems to be a consequence of its inhibition of the expressions of MAPK mRNA and MAPK protein in the brain of the rat. D.A. Spandidos 2017-06 2017-04-10 /pmc/articles/PMC5450572/ /pubmed/28587340 http://dx.doi.org/10.3892/etm.2017.4322 Text en Copyright: © Song et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Song, Fu-Xia
Wang, Li
Liu, Hong
Wang, Ying-Li
Zou, Yong
Brain cell apoptosis inhibition by butylphthalide in Alzheimer's disease model in rats
title Brain cell apoptosis inhibition by butylphthalide in Alzheimer's disease model in rats
title_full Brain cell apoptosis inhibition by butylphthalide in Alzheimer's disease model in rats
title_fullStr Brain cell apoptosis inhibition by butylphthalide in Alzheimer's disease model in rats
title_full_unstemmed Brain cell apoptosis inhibition by butylphthalide in Alzheimer's disease model in rats
title_short Brain cell apoptosis inhibition by butylphthalide in Alzheimer's disease model in rats
title_sort brain cell apoptosis inhibition by butylphthalide in alzheimer's disease model in rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450572/
https://www.ncbi.nlm.nih.gov/pubmed/28587340
http://dx.doi.org/10.3892/etm.2017.4322
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