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Immunoregulatory effects of the traditional Dai prescription Yajieshaba on food allergic mice

The Dai prescription Yajieshaba is widely used in Traditional Dai Medicine to treat food allergies and intolerance. However, information on the active chemical ingredients, effects and mechanisms of action of Yajieshaba is limited. The present study aimed to elucidate the effects and underlying mech...

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Autores principales: Zhang, Guangyuan, Duan, Xiaohua, Zhang, Chao, Chen, Pu, Yu, Jie, Zheng, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450608/
https://www.ncbi.nlm.nih.gov/pubmed/28587391
http://dx.doi.org/10.3892/etm.2017.4442
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author Zhang, Guangyuan
Duan, Xiaohua
Zhang, Chao
Chen, Pu
Yu, Jie
Zheng, Jin
author_facet Zhang, Guangyuan
Duan, Xiaohua
Zhang, Chao
Chen, Pu
Yu, Jie
Zheng, Jin
author_sort Zhang, Guangyuan
collection PubMed
description The Dai prescription Yajieshaba is widely used in Traditional Dai Medicine to treat food allergies and intolerance. However, information on the active chemical ingredients, effects and mechanisms of action of Yajieshaba is limited. The present study aimed to elucidate the effects and underlying mechanisms of Yajieshaba in the treatment of food allergies. Liquid chromatography with a diode array detector was used to measure the levels of palmatine and berberine, the active ingredients of Yajieshaba. A food allergy model was established in female BALB/c mice by three injections of ovalbumin (OVA) at 0, 48, and 96 h. OVA-sensitized mice recieved no treatments (control), Yajieshaba, loratadine, palmatine or berberine. The scratching frequency, serum immunoglobulin (Ig)G, IgE, interleukin (IL)-4, IL-10, IL-17, IL-21, interferon-γ and tumor necrosis factor-α levels were assessed at 50 and 98 h. The percentage of regulatory T cells (Tregs) was evaluated by flow cytometry at 98 h. The scratching frequency induced by OVA was significantly suppressed in mice treated with loratadine, palmatine, berberine or 3.50 and 4.70 g/kg Yajieshaba. The frequency of CD4(+)CD25(+)Treg in the spleen increased from 6.80% in mice in the control group to 12.50% in mice treated with 4.70 g/kg body weight Yajieshaba. Mice treated with palmatine or 4.70 g/kg body weight Yajieshaba had increased forkhead box p3 expression compared with those in the control group. Treatment with Yajieshaba decreased the scratching frequency and increased CD4(+)CD25(+)Foxp3(+) Treg frequency in the spleen. This indicated that symptoms of allergic reaction were alleviated following Yajieshaba treatment. Palmatine was identified as one of the major active components of Yajieshaba. The present study identified the possible mechanism through which Yajieshaba treatment may alleviate food allergy symptoms.
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spelling pubmed-54506082017-06-05 Immunoregulatory effects of the traditional Dai prescription Yajieshaba on food allergic mice Zhang, Guangyuan Duan, Xiaohua Zhang, Chao Chen, Pu Yu, Jie Zheng, Jin Exp Ther Med Articles The Dai prescription Yajieshaba is widely used in Traditional Dai Medicine to treat food allergies and intolerance. However, information on the active chemical ingredients, effects and mechanisms of action of Yajieshaba is limited. The present study aimed to elucidate the effects and underlying mechanisms of Yajieshaba in the treatment of food allergies. Liquid chromatography with a diode array detector was used to measure the levels of palmatine and berberine, the active ingredients of Yajieshaba. A food allergy model was established in female BALB/c mice by three injections of ovalbumin (OVA) at 0, 48, and 96 h. OVA-sensitized mice recieved no treatments (control), Yajieshaba, loratadine, palmatine or berberine. The scratching frequency, serum immunoglobulin (Ig)G, IgE, interleukin (IL)-4, IL-10, IL-17, IL-21, interferon-γ and tumor necrosis factor-α levels were assessed at 50 and 98 h. The percentage of regulatory T cells (Tregs) was evaluated by flow cytometry at 98 h. The scratching frequency induced by OVA was significantly suppressed in mice treated with loratadine, palmatine, berberine or 3.50 and 4.70 g/kg Yajieshaba. The frequency of CD4(+)CD25(+)Treg in the spleen increased from 6.80% in mice in the control group to 12.50% in mice treated with 4.70 g/kg body weight Yajieshaba. Mice treated with palmatine or 4.70 g/kg body weight Yajieshaba had increased forkhead box p3 expression compared with those in the control group. Treatment with Yajieshaba decreased the scratching frequency and increased CD4(+)CD25(+)Foxp3(+) Treg frequency in the spleen. This indicated that symptoms of allergic reaction were alleviated following Yajieshaba treatment. Palmatine was identified as one of the major active components of Yajieshaba. The present study identified the possible mechanism through which Yajieshaba treatment may alleviate food allergy symptoms. D.A. Spandidos 2017-06 2017-05-08 /pmc/articles/PMC5450608/ /pubmed/28587391 http://dx.doi.org/10.3892/etm.2017.4442 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Guangyuan
Duan, Xiaohua
Zhang, Chao
Chen, Pu
Yu, Jie
Zheng, Jin
Immunoregulatory effects of the traditional Dai prescription Yajieshaba on food allergic mice
title Immunoregulatory effects of the traditional Dai prescription Yajieshaba on food allergic mice
title_full Immunoregulatory effects of the traditional Dai prescription Yajieshaba on food allergic mice
title_fullStr Immunoregulatory effects of the traditional Dai prescription Yajieshaba on food allergic mice
title_full_unstemmed Immunoregulatory effects of the traditional Dai prescription Yajieshaba on food allergic mice
title_short Immunoregulatory effects of the traditional Dai prescription Yajieshaba on food allergic mice
title_sort immunoregulatory effects of the traditional dai prescription yajieshaba on food allergic mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450608/
https://www.ncbi.nlm.nih.gov/pubmed/28587391
http://dx.doi.org/10.3892/etm.2017.4442
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