Cargando…

Areca nut extract protects against ovariectomy-induced osteoporosis in mice

Estrogen deficiency increases the generation of reactive oxygen species (ROS), which is a crucial pathogenic factor for osteoporosis. Areca nuts are rich in phenolics, which have high antioxidant activity. In the present study, an ovariectomy (OVX)-induced osteoporosis mouse model was used to invest...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Shuyu, Chen, Rong, Luo, Kaili, Guo, Yang, Xiao, Man, Du, Guankui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450612/
https://www.ncbi.nlm.nih.gov/pubmed/28587356
http://dx.doi.org/10.3892/etm.2017.4362
_version_ 1783240016846127104
author Li, Shuyu
Chen, Rong
Luo, Kaili
Guo, Yang
Xiao, Man
Du, Guankui
author_facet Li, Shuyu
Chen, Rong
Luo, Kaili
Guo, Yang
Xiao, Man
Du, Guankui
author_sort Li, Shuyu
collection PubMed
description Estrogen deficiency increases the generation of reactive oxygen species (ROS), which is a crucial pathogenic factor for osteoporosis. Areca nuts are rich in phenolics, which have high antioxidant activity. In the present study, an ovariectomy (OVX)-induced osteoporosis mouse model was used to investigate the protective effects of areca nut extract (ANE) on bone loss and related processes. A total of 24 8-week-old female mice were randomly divided into three groups (n=8 per group): I Sham-operated control; II, bilateral OVX; and III, bilateral OVX + ANE. Group III were treated orally with ANE at a single dose of 300 mg/kg body weight daily for 6 months. ANE supplementation for 6 months improved trabecular bone microarchitecture and significantly increased bone mineral density in the distal femur (P<0.05) compared with Group II. Furthermore, serum levels of the osteoclast differentiation-inducing factors, receptor activator of nuclear factor-κB ligand and osteoprotegerin were significantly increased and decreased, respectively (both P<0.05), in OVX mice and these effects were significantly inhibited by ANE treatment (both P<0.05). ANE supplementation also resulted in significantly decreased serum hydrogen peroxide and malondialdehyde levels compared with Group II, while the levels of glutathione and catalase activity were significantly increased (P<0.05 and P<0.01, respectively). The current study indicated that the protective effects of ANE against bone loss were mediated, at least in part, via inhibition of the release of ROS and bone resorption. These results suggested that ANE could have therapeutic value in the treatment of osteoporosis.
format Online
Article
Text
id pubmed-5450612
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-54506122017-06-05 Areca nut extract protects against ovariectomy-induced osteoporosis in mice Li, Shuyu Chen, Rong Luo, Kaili Guo, Yang Xiao, Man Du, Guankui Exp Ther Med Articles Estrogen deficiency increases the generation of reactive oxygen species (ROS), which is a crucial pathogenic factor for osteoporosis. Areca nuts are rich in phenolics, which have high antioxidant activity. In the present study, an ovariectomy (OVX)-induced osteoporosis mouse model was used to investigate the protective effects of areca nut extract (ANE) on bone loss and related processes. A total of 24 8-week-old female mice were randomly divided into three groups (n=8 per group): I Sham-operated control; II, bilateral OVX; and III, bilateral OVX + ANE. Group III were treated orally with ANE at a single dose of 300 mg/kg body weight daily for 6 months. ANE supplementation for 6 months improved trabecular bone microarchitecture and significantly increased bone mineral density in the distal femur (P<0.05) compared with Group II. Furthermore, serum levels of the osteoclast differentiation-inducing factors, receptor activator of nuclear factor-κB ligand and osteoprotegerin were significantly increased and decreased, respectively (both P<0.05), in OVX mice and these effects were significantly inhibited by ANE treatment (both P<0.05). ANE supplementation also resulted in significantly decreased serum hydrogen peroxide and malondialdehyde levels compared with Group II, while the levels of glutathione and catalase activity were significantly increased (P<0.05 and P<0.01, respectively). The current study indicated that the protective effects of ANE against bone loss were mediated, at least in part, via inhibition of the release of ROS and bone resorption. These results suggested that ANE could have therapeutic value in the treatment of osteoporosis. D.A. Spandidos 2017-06 2017-04-19 /pmc/articles/PMC5450612/ /pubmed/28587356 http://dx.doi.org/10.3892/etm.2017.4362 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Shuyu
Chen, Rong
Luo, Kaili
Guo, Yang
Xiao, Man
Du, Guankui
Areca nut extract protects against ovariectomy-induced osteoporosis in mice
title Areca nut extract protects against ovariectomy-induced osteoporosis in mice
title_full Areca nut extract protects against ovariectomy-induced osteoporosis in mice
title_fullStr Areca nut extract protects against ovariectomy-induced osteoporosis in mice
title_full_unstemmed Areca nut extract protects against ovariectomy-induced osteoporosis in mice
title_short Areca nut extract protects against ovariectomy-induced osteoporosis in mice
title_sort areca nut extract protects against ovariectomy-induced osteoporosis in mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450612/
https://www.ncbi.nlm.nih.gov/pubmed/28587356
http://dx.doi.org/10.3892/etm.2017.4362
work_keys_str_mv AT lishuyu arecanutextractprotectsagainstovariectomyinducedosteoporosisinmice
AT chenrong arecanutextractprotectsagainstovariectomyinducedosteoporosisinmice
AT luokaili arecanutextractprotectsagainstovariectomyinducedosteoporosisinmice
AT guoyang arecanutextractprotectsagainstovariectomyinducedosteoporosisinmice
AT xiaoman arecanutextractprotectsagainstovariectomyinducedosteoporosisinmice
AT duguankui arecanutextractprotectsagainstovariectomyinducedosteoporosisinmice