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Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin
Research about antibody specificity spectra was conducted to develop single-specific antibodies or broad-specific antibodies. Aflatoxins, as one class of high-toxicity mycotoxins, were selected as the research targets to investigate the effect of the immunogen dose on antibody specificity spectra. F...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450720/ https://www.ncbi.nlm.nih.gov/pubmed/28534846 http://dx.doi.org/10.3390/toxins9050172 |
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author | Li, Peiwu Wu, Jing Zhang, Li Fan, Zhiyong Yu, Tingting Jiang, Feng Tang, Xiaoqian Zhang, Zhaowei Zhang, Wen Zhang, Qi |
author_facet | Li, Peiwu Wu, Jing Zhang, Li Fan, Zhiyong Yu, Tingting Jiang, Feng Tang, Xiaoqian Zhang, Zhaowei Zhang, Wen Zhang, Qi |
author_sort | Li, Peiwu |
collection | PubMed |
description | Research about antibody specificity spectra was conducted to develop single-specific antibodies or broad-specific antibodies. Aflatoxins, as one class of high-toxicity mycotoxins, were selected as the research targets to investigate the effect of the immunogen dose on antibody specificity spectra. For this aim, 16 monoclonal antibodies were induced by low or high doses of aflatoxin B(1)-BSA, and 34 monoclonal antibodies were induced by low or high doses of aflatoxin M(1)-BSA. The specificities of the antibodies induced, whether by aflatoxin B(1) conjugate or aflatoxin M(1) conjugate, indicated that the low dose of the immunogen induced a narrow spectrum of antibody specificity, while the high dose of the immunogen showed an advantage to form a broad spectrum of antibody specificity. Therefore, this report provides important information for the development of new antibodies against small molecules like aflatoxins. |
format | Online Article Text |
id | pubmed-5450720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54507202017-06-05 Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin Li, Peiwu Wu, Jing Zhang, Li Fan, Zhiyong Yu, Tingting Jiang, Feng Tang, Xiaoqian Zhang, Zhaowei Zhang, Wen Zhang, Qi Toxins (Basel) Article Research about antibody specificity spectra was conducted to develop single-specific antibodies or broad-specific antibodies. Aflatoxins, as one class of high-toxicity mycotoxins, were selected as the research targets to investigate the effect of the immunogen dose on antibody specificity spectra. For this aim, 16 monoclonal antibodies were induced by low or high doses of aflatoxin B(1)-BSA, and 34 monoclonal antibodies were induced by low or high doses of aflatoxin M(1)-BSA. The specificities of the antibodies induced, whether by aflatoxin B(1) conjugate or aflatoxin M(1) conjugate, indicated that the low dose of the immunogen induced a narrow spectrum of antibody specificity, while the high dose of the immunogen showed an advantage to form a broad spectrum of antibody specificity. Therefore, this report provides important information for the development of new antibodies against small molecules like aflatoxins. MDPI 2017-05-19 /pmc/articles/PMC5450720/ /pubmed/28534846 http://dx.doi.org/10.3390/toxins9050172 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Peiwu Wu, Jing Zhang, Li Fan, Zhiyong Yu, Tingting Jiang, Feng Tang, Xiaoqian Zhang, Zhaowei Zhang, Wen Zhang, Qi Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin |
title | Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin |
title_full | Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin |
title_fullStr | Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin |
title_full_unstemmed | Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin |
title_short | Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin |
title_sort | doses of immunogen contribute to specificity spectrums of antibodies against aflatoxin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450720/ https://www.ncbi.nlm.nih.gov/pubmed/28534846 http://dx.doi.org/10.3390/toxins9050172 |
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