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DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expre...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Parasitology and Tropical Medicine
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450953/ https://www.ncbi.nlm.nih.gov/pubmed/28506032 http://dx.doi.org/10.3347/kjp.2017.55.2.115 |
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author | Moon, Eun-Kyung Hong, Yeonchul Lee, Hae-Ahm Quan, Fu-Shi Kong, Hyun-Hee |
author_facet | Moon, Eun-Kyung Hong, Yeonchul Lee, Hae-Ahm Quan, Fu-Shi Kong, Hyun-Hee |
author_sort | Moon, Eun-Kyung |
collection | PubMed |
description | Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1–3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation. |
format | Online Article Text |
id | pubmed-5450953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Korean Society for Parasitology and Tropical Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-54509532017-06-01 DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation Moon, Eun-Kyung Hong, Yeonchul Lee, Hae-Ahm Quan, Fu-Shi Kong, Hyun-Hee Korean J Parasitol Original Article Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1–3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation. The Korean Society for Parasitology and Tropical Medicine 2017-04 2017-04-30 /pmc/articles/PMC5450953/ /pubmed/28506032 http://dx.doi.org/10.3347/kjp.2017.55.2.115 Text en Copyright © 2017 by The Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Moon, Eun-Kyung Hong, Yeonchul Lee, Hae-Ahm Quan, Fu-Shi Kong, Hyun-Hee DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation |
title | DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation |
title_full | DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation |
title_fullStr | DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation |
title_full_unstemmed | DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation |
title_short | DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation |
title_sort | dna methylation of gene expression in acanthamoeba castellanii encystation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450953/ https://www.ncbi.nlm.nih.gov/pubmed/28506032 http://dx.doi.org/10.3347/kjp.2017.55.2.115 |
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