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Bacteriophage–prokaryote dynamics and interaction within anaerobic digestion processes across time and space

BACKGROUND: Bacteriophage–prokaryote dynamics and interaction are believed to be important in governing microbiome composition and ecosystem functions, yet our limited knowledge of the spatial and temporal variation in phage and prokaryotic community compositions precludes accurate assessment of the...

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Autores principales: Zhang, Junyu, Gao, Qun, Zhang, Qiuting, Wang, Tengxu, Yue, Haowei, Wu, Linwei, Shi, Jason, Qin, Ziyan, Zhou, Jizhong, Zuo, Jiane, Yang, Yunfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5452288/
https://www.ncbi.nlm.nih.gov/pubmed/28569210
http://dx.doi.org/10.1186/s40168-017-0272-8
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author Zhang, Junyu
Gao, Qun
Zhang, Qiuting
Wang, Tengxu
Yue, Haowei
Wu, Linwei
Shi, Jason
Qin, Ziyan
Zhou, Jizhong
Zuo, Jiane
Yang, Yunfeng
author_facet Zhang, Junyu
Gao, Qun
Zhang, Qiuting
Wang, Tengxu
Yue, Haowei
Wu, Linwei
Shi, Jason
Qin, Ziyan
Zhou, Jizhong
Zuo, Jiane
Yang, Yunfeng
author_sort Zhang, Junyu
collection PubMed
description BACKGROUND: Bacteriophage–prokaryote dynamics and interaction are believed to be important in governing microbiome composition and ecosystem functions, yet our limited knowledge of the spatial and temporal variation in phage and prokaryotic community compositions precludes accurate assessment of their roles and impacts. Anaerobic digesters are ideal model systems to examine phage–host interaction, owing to easy access, stable operation, nutrient-rich environment, and consequently enormous numbers of phages and prokaryotic cells. RESULTS: Equipped with high-throughput, cutting-edge environmental genomics techniques, we examined phage and prokaryotic community composition of four anaerobic digesters in full-scale wastewater treatment plants across China. Despite the relatively stable process performance in biogas production, phage and prokaryotic groups fluctuated monthly over a year of study period, showing significant correlations between those two groups at the α- and β-diversity levels. Strikingly, phages explained 40.6% of total variations of the prokaryotic community composition, much higher than the explanatory power by abiotic factors (14.5%). Consequently, phages were significantly (P < 0.010) linked to parameters related to process performance including biogas production and volatile solid concentrations. Association network analyses showed phage–prokaryote pairs were shallowly conserved since they were detected only within small viral clades. CONCLUSIONS: Those results collectively demonstrate phages as a major biotic factor in controlling prokaryotic composition and process performance. Therefore, phages may play a larger role in shaping prokaryotic community dynamics and process performance of anaerobic digesters than currently appreciated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40168-017-0272-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-54522882017-06-01 Bacteriophage–prokaryote dynamics and interaction within anaerobic digestion processes across time and space Zhang, Junyu Gao, Qun Zhang, Qiuting Wang, Tengxu Yue, Haowei Wu, Linwei Shi, Jason Qin, Ziyan Zhou, Jizhong Zuo, Jiane Yang, Yunfeng Microbiome Research BACKGROUND: Bacteriophage–prokaryote dynamics and interaction are believed to be important in governing microbiome composition and ecosystem functions, yet our limited knowledge of the spatial and temporal variation in phage and prokaryotic community compositions precludes accurate assessment of their roles and impacts. Anaerobic digesters are ideal model systems to examine phage–host interaction, owing to easy access, stable operation, nutrient-rich environment, and consequently enormous numbers of phages and prokaryotic cells. RESULTS: Equipped with high-throughput, cutting-edge environmental genomics techniques, we examined phage and prokaryotic community composition of four anaerobic digesters in full-scale wastewater treatment plants across China. Despite the relatively stable process performance in biogas production, phage and prokaryotic groups fluctuated monthly over a year of study period, showing significant correlations between those two groups at the α- and β-diversity levels. Strikingly, phages explained 40.6% of total variations of the prokaryotic community composition, much higher than the explanatory power by abiotic factors (14.5%). Consequently, phages were significantly (P < 0.010) linked to parameters related to process performance including biogas production and volatile solid concentrations. Association network analyses showed phage–prokaryote pairs were shallowly conserved since they were detected only within small viral clades. CONCLUSIONS: Those results collectively demonstrate phages as a major biotic factor in controlling prokaryotic composition and process performance. Therefore, phages may play a larger role in shaping prokaryotic community dynamics and process performance of anaerobic digesters than currently appreciated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40168-017-0272-8) contains supplementary material, which is available to authorized users. BioMed Central 2017-05-31 /pmc/articles/PMC5452288/ /pubmed/28569210 http://dx.doi.org/10.1186/s40168-017-0272-8 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Junyu
Gao, Qun
Zhang, Qiuting
Wang, Tengxu
Yue, Haowei
Wu, Linwei
Shi, Jason
Qin, Ziyan
Zhou, Jizhong
Zuo, Jiane
Yang, Yunfeng
Bacteriophage–prokaryote dynamics and interaction within anaerobic digestion processes across time and space
title Bacteriophage–prokaryote dynamics and interaction within anaerobic digestion processes across time and space
title_full Bacteriophage–prokaryote dynamics and interaction within anaerobic digestion processes across time and space
title_fullStr Bacteriophage–prokaryote dynamics and interaction within anaerobic digestion processes across time and space
title_full_unstemmed Bacteriophage–prokaryote dynamics and interaction within anaerobic digestion processes across time and space
title_short Bacteriophage–prokaryote dynamics and interaction within anaerobic digestion processes across time and space
title_sort bacteriophage–prokaryote dynamics and interaction within anaerobic digestion processes across time and space
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5452288/
https://www.ncbi.nlm.nih.gov/pubmed/28569210
http://dx.doi.org/10.1186/s40168-017-0272-8
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