Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

BACKGROUND: Molecular tools for detecting malaria-infected mosquitoes with improved practicality, sensitivity and specificity, and high-throughput are required. A common PCR technique used to detect mosquitoes infected with Plasmodium spp. is a nested PCR assay based on the 18s-rRNA gene. However, t...

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Detalles Bibliográficos
Autores principales: Echeverry, Diego F., Deason, Nicholas A., Makuru, Victoria, Davidson, Jenna, Xiao, Honglin, Niedbalski, Julie, Yu, Xiaoyu, Stevenson, Jennifer C., Bugoro, Hugo, Aparaimo, Allan, Reuben, Hedrick, Cooper, Robert, Burkot, Thomas R., Russell, Tanya L., Collins, Frank H., Lobo, Neil F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5452387/
https://www.ncbi.nlm.nih.gov/pubmed/28569159
http://dx.doi.org/10.1186/s12936-017-1881-1

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