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Cytomorphometric Analysis on the Effects of Components of Orthodontic Appliances on the Epithelial Cells of the Buccal Mucosa
AIM: The aim of the present study was to evaluate the effect of fixed orthodontic appliances on the epithelial cells of buccal mucosa in patients undergoing orthodontic treatment. MATERIALS AND METHODS: The study group included 30 healthy patients who were advised orthodontic treatment. Applying ste...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5452568/ https://www.ncbi.nlm.nih.gov/pubmed/28584785 http://dx.doi.org/10.4103/jispcd.JISPCD_58_17 |
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author | Francis, P. G. Thomas, Mathai Antony, Vincy Shaloob, Muhammed Hassan, K. Jubin Roshan, Gazanafer |
author_facet | Francis, P. G. Thomas, Mathai Antony, Vincy Shaloob, Muhammed Hassan, K. Jubin Roshan, Gazanafer |
author_sort | Francis, P. G. |
collection | PubMed |
description | AIM: The aim of the present study was to evaluate the effect of fixed orthodontic appliances on the epithelial cells of buccal mucosa in patients undergoing orthodontic treatment. MATERIALS AND METHODS: The study group included 30 healthy patients who were advised orthodontic treatment. Applying sterile cement spatula, scrapping of exfoliated buccal epithelial cells was performed from the middle part of the inner cheeks before starting the orthodontic treatment and at 1(st) week, 2(nd) week, 1 month, and 45 days after the treatment, followed by smearing and staining with Papanicolaou stain. Light microscope was used to score micronuclei, and independent two-tailed t-test was used for statistical analysis. RESULTS: Micronuclei were assessed during the various stages of treatment and were recorded accordingly. At 1 week, there was increase in micronuclei number compared to before starting the treatment (day 0) and at 15(th) day; 30(th) day showed decrease in number compared to 1 week but increase compared to day 0. The results of day 45 were almost equal to day 0 with a significant P value (P < 0.001). CONCLUSION: Fixed orthodontic appliances induce increased micronuclei frequency, especially in the first weeks of treatment, however, these genotoxic effects tend to approach baseline levels in later period. |
format | Online Article Text |
id | pubmed-5452568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-54525682017-06-05 Cytomorphometric Analysis on the Effects of Components of Orthodontic Appliances on the Epithelial Cells of the Buccal Mucosa Francis, P. G. Thomas, Mathai Antony, Vincy Shaloob, Muhammed Hassan, K. Jubin Roshan, Gazanafer J Int Soc Prev Community Dent Original Article AIM: The aim of the present study was to evaluate the effect of fixed orthodontic appliances on the epithelial cells of buccal mucosa in patients undergoing orthodontic treatment. MATERIALS AND METHODS: The study group included 30 healthy patients who were advised orthodontic treatment. Applying sterile cement spatula, scrapping of exfoliated buccal epithelial cells was performed from the middle part of the inner cheeks before starting the orthodontic treatment and at 1(st) week, 2(nd) week, 1 month, and 45 days after the treatment, followed by smearing and staining with Papanicolaou stain. Light microscope was used to score micronuclei, and independent two-tailed t-test was used for statistical analysis. RESULTS: Micronuclei were assessed during the various stages of treatment and were recorded accordingly. At 1 week, there was increase in micronuclei number compared to before starting the treatment (day 0) and at 15(th) day; 30(th) day showed decrease in number compared to 1 week but increase compared to day 0. The results of day 45 were almost equal to day 0 with a significant P value (P < 0.001). CONCLUSION: Fixed orthodontic appliances induce increased micronuclei frequency, especially in the first weeks of treatment, however, these genotoxic effects tend to approach baseline levels in later period. Medknow Publications & Media Pvt Ltd 2017 2017-05-22 /pmc/articles/PMC5452568/ /pubmed/28584785 http://dx.doi.org/10.4103/jispcd.JISPCD_58_17 Text en Copyright: © 2017 Journal of International Society of Preventive and Community Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Francis, P. G. Thomas, Mathai Antony, Vincy Shaloob, Muhammed Hassan, K. Jubin Roshan, Gazanafer Cytomorphometric Analysis on the Effects of Components of Orthodontic Appliances on the Epithelial Cells of the Buccal Mucosa |
title | Cytomorphometric Analysis on the Effects of Components of Orthodontic Appliances on the Epithelial Cells of the Buccal Mucosa |
title_full | Cytomorphometric Analysis on the Effects of Components of Orthodontic Appliances on the Epithelial Cells of the Buccal Mucosa |
title_fullStr | Cytomorphometric Analysis on the Effects of Components of Orthodontic Appliances on the Epithelial Cells of the Buccal Mucosa |
title_full_unstemmed | Cytomorphometric Analysis on the Effects of Components of Orthodontic Appliances on the Epithelial Cells of the Buccal Mucosa |
title_short | Cytomorphometric Analysis on the Effects of Components of Orthodontic Appliances on the Epithelial Cells of the Buccal Mucosa |
title_sort | cytomorphometric analysis on the effects of components of orthodontic appliances on the epithelial cells of the buccal mucosa |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5452568/ https://www.ncbi.nlm.nih.gov/pubmed/28584785 http://dx.doi.org/10.4103/jispcd.JISPCD_58_17 |
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