Fluorescence Sensing of the Interaction between Biomembranes with Different Lipid Composition and Endocrine Disrupting Chemicals

Fluorescence sensing of the interaction between biomembranes with different lipid composition and endocrine disrupting chemicals (EDCs) was carried out by using a liposome-encapsulating fluorescence dye (carboxyfluorescein (CF)-liposome). We detected a significant increase in fluorescence intensity in CF-liposome solutions due to the leakage of fluorescence caused by the interaction of EDCs with the biomembranes of liposomes. The temporal increases in fluorescent were significantly different among the lipid compositions of CF-liposome and the EDCs. Results were considered by summarizing the interactions in radar charts and by showing the pattern of interaction of each EDC. Each chart showed a dissimilar pattern reflecting the complexity of the biomembrane-EDC interaction. The results indicate that this fluorescence sensing could be useful to evaluate the interaction.