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SCYL1 does not regulate REST expression and turnover
A recent study identified SCYL1 as one of the components of the oncogenic STP axis, which promotes triple-negative breast cancer by regulating degradation of the REST tumor suppressor. Contrary to the findings of that study, herein we show by using 3 distinct genetic approaches that SCYL1 does not r...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453557/ https://www.ncbi.nlm.nih.gov/pubmed/28570664 http://dx.doi.org/10.1371/journal.pone.0178680 |
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author | Gingras, Sebastien Kuliyev, Emin Pelletier, Stéphane |
author_facet | Gingras, Sebastien Kuliyev, Emin Pelletier, Stéphane |
author_sort | Gingras, Sebastien |
collection | PubMed |
description | A recent study identified SCYL1 as one of the components of the oncogenic STP axis, which promotes triple-negative breast cancer by regulating degradation of the REST tumor suppressor. Contrary to the findings of that study, herein we show by using 3 distinct genetic approaches that SCYL1 does not regulate REST turnover. Specifically, REST protein levels and turnover were identical in Scyl1+/+ and Scyl1-/- mouse embryonic fibroblasts. Similarly, targeted inactivation of SCYL1 in Hek293T cells by using CRIPSR-Cas9 technology did not affect REST steady-state level and turnover. Furthermore, RNA interference–mediated depletion of SCYL1 in Hek293T or MDA-MB-231 cells did not alter REST steady-state level and turnover. Together, our findings indicate that SCYL1 does not contribute to REST turnover and thus do not support a previous study suggesting a role for SCYL1 in mediating REST degradation. |
format | Online Article Text |
id | pubmed-5453557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54535572017-06-12 SCYL1 does not regulate REST expression and turnover Gingras, Sebastien Kuliyev, Emin Pelletier, Stéphane PLoS One Research Article A recent study identified SCYL1 as one of the components of the oncogenic STP axis, which promotes triple-negative breast cancer by regulating degradation of the REST tumor suppressor. Contrary to the findings of that study, herein we show by using 3 distinct genetic approaches that SCYL1 does not regulate REST turnover. Specifically, REST protein levels and turnover were identical in Scyl1+/+ and Scyl1-/- mouse embryonic fibroblasts. Similarly, targeted inactivation of SCYL1 in Hek293T cells by using CRIPSR-Cas9 technology did not affect REST steady-state level and turnover. Furthermore, RNA interference–mediated depletion of SCYL1 in Hek293T or MDA-MB-231 cells did not alter REST steady-state level and turnover. Together, our findings indicate that SCYL1 does not contribute to REST turnover and thus do not support a previous study suggesting a role for SCYL1 in mediating REST degradation. Public Library of Science 2017-06-01 /pmc/articles/PMC5453557/ /pubmed/28570664 http://dx.doi.org/10.1371/journal.pone.0178680 Text en © 2017 Gingras et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gingras, Sebastien Kuliyev, Emin Pelletier, Stéphane SCYL1 does not regulate REST expression and turnover |
title | SCYL1 does not regulate REST expression and turnover |
title_full | SCYL1 does not regulate REST expression and turnover |
title_fullStr | SCYL1 does not regulate REST expression and turnover |
title_full_unstemmed | SCYL1 does not regulate REST expression and turnover |
title_short | SCYL1 does not regulate REST expression and turnover |
title_sort | scyl1 does not regulate rest expression and turnover |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453557/ https://www.ncbi.nlm.nih.gov/pubmed/28570664 http://dx.doi.org/10.1371/journal.pone.0178680 |
work_keys_str_mv | AT gingrassebastien scyl1doesnotregulaterestexpressionandturnover AT kuliyevemin scyl1doesnotregulaterestexpressionandturnover AT pelletierstephane scyl1doesnotregulaterestexpressionandturnover |