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Single-protein detection in crowded molecular environments in cryo-EM images

We present an approach to study macromolecular assemblies by detecting component proteins’ characteristic high-resolution projection patterns, calculated from their known 3D structures, in single electron cryo-micrographs. Our method detects single apoferritin molecules in vitreous ice with high spe...

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Detalles Bibliográficos
Autores principales: Rickgauer, J Peter, Grigorieff, Nikolaus, Denk, Winfried
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453696/
https://www.ncbi.nlm.nih.gov/pubmed/28467302
http://dx.doi.org/10.7554/eLife.25648