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Single-protein detection in crowded molecular environments in cryo-EM images
We present an approach to study macromolecular assemblies by detecting component proteins’ characteristic high-resolution projection patterns, calculated from their known 3D structures, in single electron cryo-micrographs. Our method detects single apoferritin molecules in vitreous ice with high spe...
Autores principales: | Rickgauer, J Peter, Grigorieff, Nikolaus, Denk, Winfried |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453696/ https://www.ncbi.nlm.nih.gov/pubmed/28467302 http://dx.doi.org/10.7554/eLife.25648 |
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