The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides

The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple me...

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Detalles Bibliográficos
Autores principales: Bartnicki, Filip, Bonarek, Piotr, Kowalska, Ewa, Strzalka, Wojciech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453957/
https://www.ncbi.nlm.nih.gov/pubmed/28572575
http://dx.doi.org/10.1038/s41598-017-02432-6
Descripción
Sumario:The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple method for the purification of CPP-tagged proteins, free of additional tags, was not available to date. To fill this gap we developed the Argi system for one-step purification of proteins tagged with arginine rich CPPs.

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