The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides

The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple me...

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Autores principales: Bartnicki, Filip, Bonarek, Piotr, Kowalska, Ewa, Strzalka, Wojciech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453957/
https://www.ncbi.nlm.nih.gov/pubmed/28572575
http://dx.doi.org/10.1038/s41598-017-02432-6
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author Bartnicki, Filip
Bonarek, Piotr
Kowalska, Ewa
Strzalka, Wojciech
author_facet Bartnicki, Filip
Bonarek, Piotr
Kowalska, Ewa
Strzalka, Wojciech
author_sort Bartnicki, Filip
collection PubMed
description The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple method for the purification of CPP-tagged proteins, free of additional tags, was not available to date. To fill this gap we developed the Argi system for one-step purification of proteins tagged with arginine rich CPPs.
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spelling pubmed-54539572017-06-02 The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides Bartnicki, Filip Bonarek, Piotr Kowalska, Ewa Strzalka, Wojciech Sci Rep Article The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple method for the purification of CPP-tagged proteins, free of additional tags, was not available to date. To fill this gap we developed the Argi system for one-step purification of proteins tagged with arginine rich CPPs. Nature Publishing Group UK 2017-05-25 /pmc/articles/PMC5453957/ /pubmed/28572575 http://dx.doi.org/10.1038/s41598-017-02432-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bartnicki, Filip
Bonarek, Piotr
Kowalska, Ewa
Strzalka, Wojciech
The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_full The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_fullStr The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_full_unstemmed The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_short The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
title_sort argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453957/
https://www.ncbi.nlm.nih.gov/pubmed/28572575
http://dx.doi.org/10.1038/s41598-017-02432-6
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