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φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage
The pandemic dissemination of KPC carbapenemase-producing Klebsiella pneumoniae (KPC-KP) represents a major public health problem, given their extensive multidrug resistance profiles and primary role in causing healthcare-associated infections. This phenomenon has largely been contributed by strains...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453958/ https://www.ncbi.nlm.nih.gov/pubmed/28572684 http://dx.doi.org/10.1038/s41598-017-02788-9 |
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author | D’Andrea, Marco Maria Marmo, Pasquale Henrici De Angelis, Lucia Palmieri, Mattia Ciacci, Nagaia Di Lallo, Gustavo Demattè, Elisa Vannuccini, Elisa Lupetti, Pietro Rossolini, Gian Maria Thaller, Maria Cristina |
author_facet | D’Andrea, Marco Maria Marmo, Pasquale Henrici De Angelis, Lucia Palmieri, Mattia Ciacci, Nagaia Di Lallo, Gustavo Demattè, Elisa Vannuccini, Elisa Lupetti, Pietro Rossolini, Gian Maria Thaller, Maria Cristina |
author_sort | D’Andrea, Marco Maria |
collection | PubMed |
description | The pandemic dissemination of KPC carbapenemase-producing Klebsiella pneumoniae (KPC-KP) represents a major public health problem, given their extensive multidrug resistance profiles and primary role in causing healthcare-associated infections. This phenomenon has largely been contributed by strains of Clonal Group (CG) 258, mostly of clade II, which in some areas represent the majority of KPC-KP isolates. Here we have characterized a newly discovered lytic Podoviridae, named φBO1E, targeting KPC-KP strains of clade II lineage of CG258. Genomic sequencing revealed that φBO1E belongs to the Kp34virus genus (87% nucleotide identity to vB_KpnP_SU552A). ΦBO1E was stable over a broad pH and temperature range, exhibited strict specificity for K. pneumoniae strains of clade II of CG258, and was unable to establish lysogeny. In a Galleria mellonella infection model, φBO1E was able to protect larvae from death following infection with KPC-KP strains of clade II of CG258, including one colistin resistant strain characterized by a hypermucoviscous phenotype. To our best knowledge φBO1E is the first characterized lytic phage targeting K. pneumoniae strains of this pandemic clonal lineage. As such, it could be of potential interest to develop new agents for treatment of KPC-KP infections and for decolonization of subjects chronically colonized by these resistant superbugs. |
format | Online Article Text |
id | pubmed-5453958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54539582017-06-02 φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage D’Andrea, Marco Maria Marmo, Pasquale Henrici De Angelis, Lucia Palmieri, Mattia Ciacci, Nagaia Di Lallo, Gustavo Demattè, Elisa Vannuccini, Elisa Lupetti, Pietro Rossolini, Gian Maria Thaller, Maria Cristina Sci Rep Article The pandemic dissemination of KPC carbapenemase-producing Klebsiella pneumoniae (KPC-KP) represents a major public health problem, given their extensive multidrug resistance profiles and primary role in causing healthcare-associated infections. This phenomenon has largely been contributed by strains of Clonal Group (CG) 258, mostly of clade II, which in some areas represent the majority of KPC-KP isolates. Here we have characterized a newly discovered lytic Podoviridae, named φBO1E, targeting KPC-KP strains of clade II lineage of CG258. Genomic sequencing revealed that φBO1E belongs to the Kp34virus genus (87% nucleotide identity to vB_KpnP_SU552A). ΦBO1E was stable over a broad pH and temperature range, exhibited strict specificity for K. pneumoniae strains of clade II of CG258, and was unable to establish lysogeny. In a Galleria mellonella infection model, φBO1E was able to protect larvae from death following infection with KPC-KP strains of clade II of CG258, including one colistin resistant strain characterized by a hypermucoviscous phenotype. To our best knowledge φBO1E is the first characterized lytic phage targeting K. pneumoniae strains of this pandemic clonal lineage. As such, it could be of potential interest to develop new agents for treatment of KPC-KP infections and for decolonization of subjects chronically colonized by these resistant superbugs. Nature Publishing Group UK 2017-06-01 /pmc/articles/PMC5453958/ /pubmed/28572684 http://dx.doi.org/10.1038/s41598-017-02788-9 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article D’Andrea, Marco Maria Marmo, Pasquale Henrici De Angelis, Lucia Palmieri, Mattia Ciacci, Nagaia Di Lallo, Gustavo Demattè, Elisa Vannuccini, Elisa Lupetti, Pietro Rossolini, Gian Maria Thaller, Maria Cristina φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage |
title | φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage |
title_full | φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage |
title_fullStr | φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage |
title_full_unstemmed | φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage |
title_short | φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage |
title_sort | φbo1e, a newly discovered lytic bacteriophage targeting carbapenemase-producing klebsiella pneumoniae of the pandemic clonal group 258 clade ii lineage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453958/ https://www.ncbi.nlm.nih.gov/pubmed/28572684 http://dx.doi.org/10.1038/s41598-017-02788-9 |
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