Cargando…
Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress
Lactobacillus acetotolerans is a major microbe contributing to the Chinese liquor fermentation with unknown function. It can be grown well in a high concentration of ethanol. RNA sequencing (RNA-seq) was performed on L. acetotolerans F28 growing in 12% ethanol to determine important genetic mechanis...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453994/ https://www.ncbi.nlm.nih.gov/pubmed/28572611 http://dx.doi.org/10.1038/s41598-017-02975-8 |
_version_ | 1783240755146391552 |
---|---|
author | Yang, Xiaopan Teng, Kunling Zhang, Jie Wang, Fangfang Zhang, Tong Ai, Guomin Han, Peijie Bai, Fengyan Zhong, Jin |
author_facet | Yang, Xiaopan Teng, Kunling Zhang, Jie Wang, Fangfang Zhang, Tong Ai, Guomin Han, Peijie Bai, Fengyan Zhong, Jin |
author_sort | Yang, Xiaopan |
collection | PubMed |
description | Lactobacillus acetotolerans is a major microbe contributing to the Chinese liquor fermentation with unknown function. It can be grown well in a high concentration of ethanol. RNA sequencing (RNA-seq) was performed on L. acetotolerans F28 growing in 12% ethanol to determine important genetic mechanisms for both a short and long term adaption to this environment. A genome-wide transcriptional analysis revealed that the most important genetic elements for L. acetotolerans F28 grown in ethanol are related to high levels of stress response and fatty acid biosynthesis, and a reduction of amino acid transport and metabolism after both a short and long time stress. The fatty acid methyl ester analyses showed that most fatty acids were increased in L. acetotolerans F28 after exposure to ethanol while the unsaturated fatty acid octadecenoic acid (C18:1) was significantly increased. The increasing unsaturated fatty acid biosynthesis in L. acetotolerans F28 might enhance cell membrane fluidity and protect the cells against high concentration of ethanol. Overall, the transcriptome and functional analysis indicated that the elevated stress response and fatty acid biosynthesis, and the decrease of amino acid transport and metabolism might play important roles for L. acetotolerans F28 to adapt to environmental ethanol. |
format | Online Article Text |
id | pubmed-5453994 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54539942017-06-06 Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress Yang, Xiaopan Teng, Kunling Zhang, Jie Wang, Fangfang Zhang, Tong Ai, Guomin Han, Peijie Bai, Fengyan Zhong, Jin Sci Rep Article Lactobacillus acetotolerans is a major microbe contributing to the Chinese liquor fermentation with unknown function. It can be grown well in a high concentration of ethanol. RNA sequencing (RNA-seq) was performed on L. acetotolerans F28 growing in 12% ethanol to determine important genetic mechanisms for both a short and long term adaption to this environment. A genome-wide transcriptional analysis revealed that the most important genetic elements for L. acetotolerans F28 grown in ethanol are related to high levels of stress response and fatty acid biosynthesis, and a reduction of amino acid transport and metabolism after both a short and long time stress. The fatty acid methyl ester analyses showed that most fatty acids were increased in L. acetotolerans F28 after exposure to ethanol while the unsaturated fatty acid octadecenoic acid (C18:1) was significantly increased. The increasing unsaturated fatty acid biosynthesis in L. acetotolerans F28 might enhance cell membrane fluidity and protect the cells against high concentration of ethanol. Overall, the transcriptome and functional analysis indicated that the elevated stress response and fatty acid biosynthesis, and the decrease of amino acid transport and metabolism might play important roles for L. acetotolerans F28 to adapt to environmental ethanol. Nature Publishing Group UK 2017-06-01 /pmc/articles/PMC5453994/ /pubmed/28572611 http://dx.doi.org/10.1038/s41598-017-02975-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yang, Xiaopan Teng, Kunling Zhang, Jie Wang, Fangfang Zhang, Tong Ai, Guomin Han, Peijie Bai, Fengyan Zhong, Jin Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress |
title | Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress |
title_full | Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress |
title_fullStr | Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress |
title_full_unstemmed | Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress |
title_short | Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress |
title_sort | transcriptome responses of lactobacillus acetotolerans f28 to a short and long term ethanol stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453994/ https://www.ncbi.nlm.nih.gov/pubmed/28572611 http://dx.doi.org/10.1038/s41598-017-02975-8 |
work_keys_str_mv | AT yangxiaopan transcriptomeresponsesoflactobacillusacetotoleransf28toashortandlongtermethanolstress AT tengkunling transcriptomeresponsesoflactobacillusacetotoleransf28toashortandlongtermethanolstress AT zhangjie transcriptomeresponsesoflactobacillusacetotoleransf28toashortandlongtermethanolstress AT wangfangfang transcriptomeresponsesoflactobacillusacetotoleransf28toashortandlongtermethanolstress AT zhangtong transcriptomeresponsesoflactobacillusacetotoleransf28toashortandlongtermethanolstress AT aiguomin transcriptomeresponsesoflactobacillusacetotoleransf28toashortandlongtermethanolstress AT hanpeijie transcriptomeresponsesoflactobacillusacetotoleransf28toashortandlongtermethanolstress AT baifengyan transcriptomeresponsesoflactobacillusacetotoleransf28toashortandlongtermethanolstress AT zhongjin transcriptomeresponsesoflactobacillusacetotoleransf28toashortandlongtermethanolstress |