Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease

In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed...

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Lingyan, Lu, Luyao, Harvey, George, Harvey, Thomas, Rodríguez-Contreras, Adrián, Alfano, Robert R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454017/
https://www.ncbi.nlm.nih.gov/pubmed/28572632
http://dx.doi.org/10.1038/s41598-017-02673-5
_version_ 1783240761270075392
author Shi, Lingyan
Lu, Luyao
Harvey, George
Harvey, Thomas
Rodríguez-Contreras, Adrián
Alfano, Robert R.
author_facet Shi, Lingyan
Lu, Luyao
Harvey, George
Harvey, Thomas
Rodríguez-Contreras, Adrián
Alfano, Robert R.
author_sort Shi, Lingyan
collection PubMed
description In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.
format Online
Article
Text
id pubmed-5454017
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54540172017-06-06 Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease Shi, Lingyan Lu, Luyao Harvey, George Harvey, Thomas Rodríguez-Contreras, Adrián Alfano, Robert R. Sci Rep Article In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules. Nature Publishing Group UK 2017-06-01 /pmc/articles/PMC5454017/ /pubmed/28572632 http://dx.doi.org/10.1038/s41598-017-02673-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shi, Lingyan
Lu, Luyao
Harvey, George
Harvey, Thomas
Rodríguez-Contreras, Adrián
Alfano, Robert R.
Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_full Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_fullStr Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_full_unstemmed Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_short Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_sort label-free fluorescence spectroscopy for detecting key biomolecules in brain tissue from a mouse model of alzheimer’s disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454017/
https://www.ncbi.nlm.nih.gov/pubmed/28572632
http://dx.doi.org/10.1038/s41598-017-02673-5
work_keys_str_mv AT shilingyan labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease
AT luluyao labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease
AT harveygeorge labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease
AT harveythomas labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease
AT rodriguezcontrerasadrian labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease
AT alfanorobertr labelfreefluorescencespectroscopyfordetectingkeybiomoleculesinbraintissuefromamousemodelofalzheimersdisease

Ejemplares similares