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Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels
CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enh...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454077/ https://www.ncbi.nlm.nih.gov/pubmed/28626467 http://dx.doi.org/10.3389/fpls.2017.00942 |
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author | Daszkowska-Golec, Agata Skubacz, Anna Marzec, Marek Slota, Michal Kurowska, Marzena Gajecka, Monika Gajewska, Patrycja Płociniczak, Tomasz Sitko, Krzysztof Pacak, Andrzej Szweykowska-Kulinska, Zofia Szarejko, Iwona |
author_facet | Daszkowska-Golec, Agata Skubacz, Anna Marzec, Marek Slota, Michal Kurowska, Marzena Gajecka, Monika Gajewska, Patrycja Płociniczak, Tomasz Sitko, Krzysztof Pacak, Andrzej Szweykowska-Kulinska, Zofia Szarejko, Iwona |
author_sort | Daszkowska-Golec, Agata |
collection | PubMed |
description | CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the HvCBP20 gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the hvcbp20.ab mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the hvcbp20.ab mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of hvcbp20.ab mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response. |
format | Online Article Text |
id | pubmed-5454077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54540772017-06-16 Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels Daszkowska-Golec, Agata Skubacz, Anna Marzec, Marek Slota, Michal Kurowska, Marzena Gajecka, Monika Gajewska, Patrycja Płociniczak, Tomasz Sitko, Krzysztof Pacak, Andrzej Szweykowska-Kulinska, Zofia Szarejko, Iwona Front Plant Sci Plant Science CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the HvCBP20 gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the hvcbp20.ab mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the hvcbp20.ab mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of hvcbp20.ab mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response. Frontiers Media S.A. 2017-06-02 /pmc/articles/PMC5454077/ /pubmed/28626467 http://dx.doi.org/10.3389/fpls.2017.00942 Text en Copyright © 2017 Daszkowska-Golec, Skubacz, Marzec, Slota, Kurowska, Gajecka, Gajewska, Płociniczak, Sitko, Pacak, Szweykowska-Kulinska and Szarejko. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Daszkowska-Golec, Agata Skubacz, Anna Marzec, Marek Slota, Michal Kurowska, Marzena Gajecka, Monika Gajewska, Patrycja Płociniczak, Tomasz Sitko, Krzysztof Pacak, Andrzej Szweykowska-Kulinska, Zofia Szarejko, Iwona Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels |
title | Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels |
title_full | Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels |
title_fullStr | Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels |
title_full_unstemmed | Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels |
title_short | Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels |
title_sort | mutation in hvcbp20 (cap binding protein 20) adapts barley to drought stress at phenotypic and transcriptomic levels |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454077/ https://www.ncbi.nlm.nih.gov/pubmed/28626467 http://dx.doi.org/10.3389/fpls.2017.00942 |
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