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Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)
Data from EV-D68-infected patients demonstrate that pathological changes in the lower respiratory tract are principally characterized by severe respiratory illness in children and acute flaccid myelitis. However, lack of a suitable animal model for EV-D68 infection has limited the study on the patho...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454417/ https://www.ncbi.nlm.nih.gov/pubmed/28489053 http://dx.doi.org/10.3390/v9050104 |
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author | Zheng, Hui-Wen Sun, Ming Guo, Lei Wang, Jing-Jing Song, Jie Li, Jia-Qi Li, Hong-Zhe Ning, Ruo-Tong Yang, Ze-Ning Fan, Hai-Tao He, Zhan-Long Liu, Long-Ding |
author_facet | Zheng, Hui-Wen Sun, Ming Guo, Lei Wang, Jing-Jing Song, Jie Li, Jia-Qi Li, Hong-Zhe Ning, Ruo-Tong Yang, Ze-Ning Fan, Hai-Tao He, Zhan-Long Liu, Long-Ding |
author_sort | Zheng, Hui-Wen |
collection | PubMed |
description | Data from EV-D68-infected patients demonstrate that pathological changes in the lower respiratory tract are principally characterized by severe respiratory illness in children and acute flaccid myelitis. However, lack of a suitable animal model for EV-D68 infection has limited the study on the pathogenesis of this critical pathogen, and the development of a vaccine. Ferrets have been widely used to evaluate respiratory virus infections. In the current study, we used EV-D68-infected ferrets as a potential animal to identify impersonal indices, involving clinical features and histopathological changes in the upper and lower respiratory tract (URT and LRT). The research results demonstrate that the EV-D68 virus leads to minimal clinical symptoms in ferrets. According to the viral load detection in the feces, nasal, and respiratory tracts, the infection and shedding of EV-D68 in the ferret model was confirmed, and these results were supported by the EV-D68 VP1 immunofluorescence confocal imaging with α2,6-linked sialic acid (SA) in lung tissues. Furthermore, we detected the inflammatory cytokine/chemokine expression level, which implied high expression levels of interleukin (IL)-1a, IL-8, IL-5, IL-12, IL-13, and IL-17a in the lungs. These data indicate that systemic observation of responses following infection with EV-D68 in ferrets could be used as a model for EV-D68 infection and pathogenesis. |
format | Online Article Text |
id | pubmed-5454417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54544172017-06-08 Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo) Zheng, Hui-Wen Sun, Ming Guo, Lei Wang, Jing-Jing Song, Jie Li, Jia-Qi Li, Hong-Zhe Ning, Ruo-Tong Yang, Ze-Ning Fan, Hai-Tao He, Zhan-Long Liu, Long-Ding Viruses Article Data from EV-D68-infected patients demonstrate that pathological changes in the lower respiratory tract are principally characterized by severe respiratory illness in children and acute flaccid myelitis. However, lack of a suitable animal model for EV-D68 infection has limited the study on the pathogenesis of this critical pathogen, and the development of a vaccine. Ferrets have been widely used to evaluate respiratory virus infections. In the current study, we used EV-D68-infected ferrets as a potential animal to identify impersonal indices, involving clinical features and histopathological changes in the upper and lower respiratory tract (URT and LRT). The research results demonstrate that the EV-D68 virus leads to minimal clinical symptoms in ferrets. According to the viral load detection in the feces, nasal, and respiratory tracts, the infection and shedding of EV-D68 in the ferret model was confirmed, and these results were supported by the EV-D68 VP1 immunofluorescence confocal imaging with α2,6-linked sialic acid (SA) in lung tissues. Furthermore, we detected the inflammatory cytokine/chemokine expression level, which implied high expression levels of interleukin (IL)-1a, IL-8, IL-5, IL-12, IL-13, and IL-17a in the lungs. These data indicate that systemic observation of responses following infection with EV-D68 in ferrets could be used as a model for EV-D68 infection and pathogenesis. MDPI 2017-05-10 /pmc/articles/PMC5454417/ /pubmed/28489053 http://dx.doi.org/10.3390/v9050104 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zheng, Hui-Wen Sun, Ming Guo, Lei Wang, Jing-Jing Song, Jie Li, Jia-Qi Li, Hong-Zhe Ning, Ruo-Tong Yang, Ze-Ning Fan, Hai-Tao He, Zhan-Long Liu, Long-Ding Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo) |
title | Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo) |
title_full | Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo) |
title_fullStr | Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo) |
title_full_unstemmed | Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo) |
title_short | Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo) |
title_sort | nasal infection of enterovirus d68 leading to lower respiratory tract pathogenesis in ferrets (mustela putorius furo) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454417/ https://www.ncbi.nlm.nih.gov/pubmed/28489053 http://dx.doi.org/10.3390/v9050104 |
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