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Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)

Data from EV-D68-infected patients demonstrate that pathological changes in the lower respiratory tract are principally characterized by severe respiratory illness in children and acute flaccid myelitis. However, lack of a suitable animal model for EV-D68 infection has limited the study on the patho...

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Autores principales: Zheng, Hui-Wen, Sun, Ming, Guo, Lei, Wang, Jing-Jing, Song, Jie, Li, Jia-Qi, Li, Hong-Zhe, Ning, Ruo-Tong, Yang, Ze-Ning, Fan, Hai-Tao, He, Zhan-Long, Liu, Long-Ding
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454417/
https://www.ncbi.nlm.nih.gov/pubmed/28489053
http://dx.doi.org/10.3390/v9050104
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author Zheng, Hui-Wen
Sun, Ming
Guo, Lei
Wang, Jing-Jing
Song, Jie
Li, Jia-Qi
Li, Hong-Zhe
Ning, Ruo-Tong
Yang, Ze-Ning
Fan, Hai-Tao
He, Zhan-Long
Liu, Long-Ding
author_facet Zheng, Hui-Wen
Sun, Ming
Guo, Lei
Wang, Jing-Jing
Song, Jie
Li, Jia-Qi
Li, Hong-Zhe
Ning, Ruo-Tong
Yang, Ze-Ning
Fan, Hai-Tao
He, Zhan-Long
Liu, Long-Ding
author_sort Zheng, Hui-Wen
collection PubMed
description Data from EV-D68-infected patients demonstrate that pathological changes in the lower respiratory tract are principally characterized by severe respiratory illness in children and acute flaccid myelitis. However, lack of a suitable animal model for EV-D68 infection has limited the study on the pathogenesis of this critical pathogen, and the development of a vaccine. Ferrets have been widely used to evaluate respiratory virus infections. In the current study, we used EV-D68-infected ferrets as a potential animal to identify impersonal indices, involving clinical features and histopathological changes in the upper and lower respiratory tract (URT and LRT). The research results demonstrate that the EV-D68 virus leads to minimal clinical symptoms in ferrets. According to the viral load detection in the feces, nasal, and respiratory tracts, the infection and shedding of EV-D68 in the ferret model was confirmed, and these results were supported by the EV-D68 VP1 immunofluorescence confocal imaging with α2,6-linked sialic acid (SA) in lung tissues. Furthermore, we detected the inflammatory cytokine/chemokine expression level, which implied high expression levels of interleukin (IL)-1a, IL-8, IL-5, IL-12, IL-13, and IL-17a in the lungs. These data indicate that systemic observation of responses following infection with EV-D68 in ferrets could be used as a model for EV-D68 infection and pathogenesis.
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spelling pubmed-54544172017-06-08 Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo) Zheng, Hui-Wen Sun, Ming Guo, Lei Wang, Jing-Jing Song, Jie Li, Jia-Qi Li, Hong-Zhe Ning, Ruo-Tong Yang, Ze-Ning Fan, Hai-Tao He, Zhan-Long Liu, Long-Ding Viruses Article Data from EV-D68-infected patients demonstrate that pathological changes in the lower respiratory tract are principally characterized by severe respiratory illness in children and acute flaccid myelitis. However, lack of a suitable animal model for EV-D68 infection has limited the study on the pathogenesis of this critical pathogen, and the development of a vaccine. Ferrets have been widely used to evaluate respiratory virus infections. In the current study, we used EV-D68-infected ferrets as a potential animal to identify impersonal indices, involving clinical features and histopathological changes in the upper and lower respiratory tract (URT and LRT). The research results demonstrate that the EV-D68 virus leads to minimal clinical symptoms in ferrets. According to the viral load detection in the feces, nasal, and respiratory tracts, the infection and shedding of EV-D68 in the ferret model was confirmed, and these results were supported by the EV-D68 VP1 immunofluorescence confocal imaging with α2,6-linked sialic acid (SA) in lung tissues. Furthermore, we detected the inflammatory cytokine/chemokine expression level, which implied high expression levels of interleukin (IL)-1a, IL-8, IL-5, IL-12, IL-13, and IL-17a in the lungs. These data indicate that systemic observation of responses following infection with EV-D68 in ferrets could be used as a model for EV-D68 infection and pathogenesis. MDPI 2017-05-10 /pmc/articles/PMC5454417/ /pubmed/28489053 http://dx.doi.org/10.3390/v9050104 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Hui-Wen
Sun, Ming
Guo, Lei
Wang, Jing-Jing
Song, Jie
Li, Jia-Qi
Li, Hong-Zhe
Ning, Ruo-Tong
Yang, Ze-Ning
Fan, Hai-Tao
He, Zhan-Long
Liu, Long-Ding
Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)
title Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)
title_full Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)
title_fullStr Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)
title_full_unstemmed Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)
title_short Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo)
title_sort nasal infection of enterovirus d68 leading to lower respiratory tract pathogenesis in ferrets (mustela putorius furo)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454417/
https://www.ncbi.nlm.nih.gov/pubmed/28489053
http://dx.doi.org/10.3390/v9050104
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