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YKL-40-Induced Inhibition of miR-590-3p Promotes Interleukin-18 Expression and Angiogenesis of Endothelial Progenitor Cells

YKL-40, also known as human cartilage glycoprotein-39 or chitinase-3-like-1, is a pro-inflammatory protein that is highly expressed in rheumatoid arthritis (RA) patients. Angiogenesis is a critical step in the pathogenesis of RA, promoting the infiltration of inflammatory cells into joints and provi...

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Autores principales: Li, Te-Mao, Liu, Shan-Chi, Huang, Ya-Hsin, Huang, Chien-Chung, Hsu, Chin-Jung, Tsai, Chun-Hao, Wang, Shih-Wei, Tang, Chih-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454833/
https://www.ncbi.nlm.nih.gov/pubmed/28448439
http://dx.doi.org/10.3390/ijms18050920
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author Li, Te-Mao
Liu, Shan-Chi
Huang, Ya-Hsin
Huang, Chien-Chung
Hsu, Chin-Jung
Tsai, Chun-Hao
Wang, Shih-Wei
Tang, Chih-Hsin
author_facet Li, Te-Mao
Liu, Shan-Chi
Huang, Ya-Hsin
Huang, Chien-Chung
Hsu, Chin-Jung
Tsai, Chun-Hao
Wang, Shih-Wei
Tang, Chih-Hsin
author_sort Li, Te-Mao
collection PubMed
description YKL-40, also known as human cartilage glycoprotein-39 or chitinase-3-like-1, is a pro-inflammatory protein that is highly expressed in rheumatoid arthritis (RA) patients. Angiogenesis is a critical step in the pathogenesis of RA, promoting the infiltration of inflammatory cells into joints and providing oxygen and nutrients to RA pannus. In this study, we examined the effects of YKL-40 in the production of the pro-inflammatory cytokine interleukin-18 (IL-18), and the stimulation of angiogenesis and accumulation of osteoblasts. We observed that YKL-40 induces IL-18 production in osteoblasts and thereby stimulates angiogenesis of endothelial progenitor cells (EPCs). We found that this process occurs through the suppression of miR-590-3p via the focal adhesion kinase (FAK)/PI3K/Akt signaling pathway. YKL-40 inhibition reduced angiogenesis in in vivo models of angiogenesis: the chick embryo chorioallantoic membrane (CAM) and Matrigel plug models. We report that YKL-40 stimulates IL-18 expression in osteoblasts and facilitates EPC angiogenesis.
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spelling pubmed-54548332017-06-08 YKL-40-Induced Inhibition of miR-590-3p Promotes Interleukin-18 Expression and Angiogenesis of Endothelial Progenitor Cells Li, Te-Mao Liu, Shan-Chi Huang, Ya-Hsin Huang, Chien-Chung Hsu, Chin-Jung Tsai, Chun-Hao Wang, Shih-Wei Tang, Chih-Hsin Int J Mol Sci Article YKL-40, also known as human cartilage glycoprotein-39 or chitinase-3-like-1, is a pro-inflammatory protein that is highly expressed in rheumatoid arthritis (RA) patients. Angiogenesis is a critical step in the pathogenesis of RA, promoting the infiltration of inflammatory cells into joints and providing oxygen and nutrients to RA pannus. In this study, we examined the effects of YKL-40 in the production of the pro-inflammatory cytokine interleukin-18 (IL-18), and the stimulation of angiogenesis and accumulation of osteoblasts. We observed that YKL-40 induces IL-18 production in osteoblasts and thereby stimulates angiogenesis of endothelial progenitor cells (EPCs). We found that this process occurs through the suppression of miR-590-3p via the focal adhesion kinase (FAK)/PI3K/Akt signaling pathway. YKL-40 inhibition reduced angiogenesis in in vivo models of angiogenesis: the chick embryo chorioallantoic membrane (CAM) and Matrigel plug models. We report that YKL-40 stimulates IL-18 expression in osteoblasts and facilitates EPC angiogenesis. MDPI 2017-04-27 /pmc/articles/PMC5454833/ /pubmed/28448439 http://dx.doi.org/10.3390/ijms18050920 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Te-Mao
Liu, Shan-Chi
Huang, Ya-Hsin
Huang, Chien-Chung
Hsu, Chin-Jung
Tsai, Chun-Hao
Wang, Shih-Wei
Tang, Chih-Hsin
YKL-40-Induced Inhibition of miR-590-3p Promotes Interleukin-18 Expression and Angiogenesis of Endothelial Progenitor Cells
title YKL-40-Induced Inhibition of miR-590-3p Promotes Interleukin-18 Expression and Angiogenesis of Endothelial Progenitor Cells
title_full YKL-40-Induced Inhibition of miR-590-3p Promotes Interleukin-18 Expression and Angiogenesis of Endothelial Progenitor Cells
title_fullStr YKL-40-Induced Inhibition of miR-590-3p Promotes Interleukin-18 Expression and Angiogenesis of Endothelial Progenitor Cells
title_full_unstemmed YKL-40-Induced Inhibition of miR-590-3p Promotes Interleukin-18 Expression and Angiogenesis of Endothelial Progenitor Cells
title_short YKL-40-Induced Inhibition of miR-590-3p Promotes Interleukin-18 Expression and Angiogenesis of Endothelial Progenitor Cells
title_sort ykl-40-induced inhibition of mir-590-3p promotes interleukin-18 expression and angiogenesis of endothelial progenitor cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454833/
https://www.ncbi.nlm.nih.gov/pubmed/28448439
http://dx.doi.org/10.3390/ijms18050920
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