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Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production

The formation of brain vasculature is an essential step during central nervous system development. The molecular mechanism underlying brain angiogenesis remains incompletely understood. The role of Atg7, an autophagy-related protein, in brain angiogenesis was investigated in this study. We found tha...

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Autores principales: Zhuang, Shi-Fang, Liu, Dong-Xin, Wang, Hui-Jie, Zhang, Shu-Hong, Wei, Jia-Yi, Fang, Wen-Gang, Zhang, Ke, Cao, Liu, Zhao, Wei-Dong, Chen, Yu-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454881/
https://www.ncbi.nlm.nih.gov/pubmed/28467355
http://dx.doi.org/10.3390/ijms18050968
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author Zhuang, Shi-Fang
Liu, Dong-Xin
Wang, Hui-Jie
Zhang, Shu-Hong
Wei, Jia-Yi
Fang, Wen-Gang
Zhang, Ke
Cao, Liu
Zhao, Wei-Dong
Chen, Yu-Hua
author_facet Zhuang, Shi-Fang
Liu, Dong-Xin
Wang, Hui-Jie
Zhang, Shu-Hong
Wei, Jia-Yi
Fang, Wen-Gang
Zhang, Ke
Cao, Liu
Zhao, Wei-Dong
Chen, Yu-Hua
author_sort Zhuang, Shi-Fang
collection PubMed
description The formation of brain vasculature is an essential step during central nervous system development. The molecular mechanism underlying brain angiogenesis remains incompletely understood. The role of Atg7, an autophagy-related protein, in brain angiogenesis was investigated in this study. We found that the microvessel density in mice brains with endothelial-specific knockout of Atg7 (Atg7 EKO) was significantly decreased compared to wild-type control. Consistently, in vitro angiogenesis assays showed that Atg7 knockdown impaired angiogenesis in brain microvascular endothelial cells. Further results indicated that knockdown of Atg7 reduced interleukin-6 (IL-6) expression in brain microvascular endothelial cells, which is mediated by NF-κB-dependent transcriptional control. Interestingly, exogenous IL-6 restored the impaired angiogenesis and reduced cell motility caused by Atg7 knockdown. These results demonstrated that Atg7 has proangiogenic activity in brain angiogenesis which is mediated by IL-6 production in a NF-κB-dependent manner.
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spelling pubmed-54548812017-06-08 Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production Zhuang, Shi-Fang Liu, Dong-Xin Wang, Hui-Jie Zhang, Shu-Hong Wei, Jia-Yi Fang, Wen-Gang Zhang, Ke Cao, Liu Zhao, Wei-Dong Chen, Yu-Hua Int J Mol Sci Article The formation of brain vasculature is an essential step during central nervous system development. The molecular mechanism underlying brain angiogenesis remains incompletely understood. The role of Atg7, an autophagy-related protein, in brain angiogenesis was investigated in this study. We found that the microvessel density in mice brains with endothelial-specific knockout of Atg7 (Atg7 EKO) was significantly decreased compared to wild-type control. Consistently, in vitro angiogenesis assays showed that Atg7 knockdown impaired angiogenesis in brain microvascular endothelial cells. Further results indicated that knockdown of Atg7 reduced interleukin-6 (IL-6) expression in brain microvascular endothelial cells, which is mediated by NF-κB-dependent transcriptional control. Interestingly, exogenous IL-6 restored the impaired angiogenesis and reduced cell motility caused by Atg7 knockdown. These results demonstrated that Atg7 has proangiogenic activity in brain angiogenesis which is mediated by IL-6 production in a NF-κB-dependent manner. MDPI 2017-05-03 /pmc/articles/PMC5454881/ /pubmed/28467355 http://dx.doi.org/10.3390/ijms18050968 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhuang, Shi-Fang
Liu, Dong-Xin
Wang, Hui-Jie
Zhang, Shu-Hong
Wei, Jia-Yi
Fang, Wen-Gang
Zhang, Ke
Cao, Liu
Zhao, Wei-Dong
Chen, Yu-Hua
Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production
title Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production
title_full Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production
title_fullStr Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production
title_full_unstemmed Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production
title_short Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production
title_sort atg7 regulates brain angiogenesis via nf-κb-dependent il-6 production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454881/
https://www.ncbi.nlm.nih.gov/pubmed/28467355
http://dx.doi.org/10.3390/ijms18050968
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