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Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production
The formation of brain vasculature is an essential step during central nervous system development. The molecular mechanism underlying brain angiogenesis remains incompletely understood. The role of Atg7, an autophagy-related protein, in brain angiogenesis was investigated in this study. We found tha...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454881/ https://www.ncbi.nlm.nih.gov/pubmed/28467355 http://dx.doi.org/10.3390/ijms18050968 |
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author | Zhuang, Shi-Fang Liu, Dong-Xin Wang, Hui-Jie Zhang, Shu-Hong Wei, Jia-Yi Fang, Wen-Gang Zhang, Ke Cao, Liu Zhao, Wei-Dong Chen, Yu-Hua |
author_facet | Zhuang, Shi-Fang Liu, Dong-Xin Wang, Hui-Jie Zhang, Shu-Hong Wei, Jia-Yi Fang, Wen-Gang Zhang, Ke Cao, Liu Zhao, Wei-Dong Chen, Yu-Hua |
author_sort | Zhuang, Shi-Fang |
collection | PubMed |
description | The formation of brain vasculature is an essential step during central nervous system development. The molecular mechanism underlying brain angiogenesis remains incompletely understood. The role of Atg7, an autophagy-related protein, in brain angiogenesis was investigated in this study. We found that the microvessel density in mice brains with endothelial-specific knockout of Atg7 (Atg7 EKO) was significantly decreased compared to wild-type control. Consistently, in vitro angiogenesis assays showed that Atg7 knockdown impaired angiogenesis in brain microvascular endothelial cells. Further results indicated that knockdown of Atg7 reduced interleukin-6 (IL-6) expression in brain microvascular endothelial cells, which is mediated by NF-κB-dependent transcriptional control. Interestingly, exogenous IL-6 restored the impaired angiogenesis and reduced cell motility caused by Atg7 knockdown. These results demonstrated that Atg7 has proangiogenic activity in brain angiogenesis which is mediated by IL-6 production in a NF-κB-dependent manner. |
format | Online Article Text |
id | pubmed-5454881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54548812017-06-08 Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production Zhuang, Shi-Fang Liu, Dong-Xin Wang, Hui-Jie Zhang, Shu-Hong Wei, Jia-Yi Fang, Wen-Gang Zhang, Ke Cao, Liu Zhao, Wei-Dong Chen, Yu-Hua Int J Mol Sci Article The formation of brain vasculature is an essential step during central nervous system development. The molecular mechanism underlying brain angiogenesis remains incompletely understood. The role of Atg7, an autophagy-related protein, in brain angiogenesis was investigated in this study. We found that the microvessel density in mice brains with endothelial-specific knockout of Atg7 (Atg7 EKO) was significantly decreased compared to wild-type control. Consistently, in vitro angiogenesis assays showed that Atg7 knockdown impaired angiogenesis in brain microvascular endothelial cells. Further results indicated that knockdown of Atg7 reduced interleukin-6 (IL-6) expression in brain microvascular endothelial cells, which is mediated by NF-κB-dependent transcriptional control. Interestingly, exogenous IL-6 restored the impaired angiogenesis and reduced cell motility caused by Atg7 knockdown. These results demonstrated that Atg7 has proangiogenic activity in brain angiogenesis which is mediated by IL-6 production in a NF-κB-dependent manner. MDPI 2017-05-03 /pmc/articles/PMC5454881/ /pubmed/28467355 http://dx.doi.org/10.3390/ijms18050968 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhuang, Shi-Fang Liu, Dong-Xin Wang, Hui-Jie Zhang, Shu-Hong Wei, Jia-Yi Fang, Wen-Gang Zhang, Ke Cao, Liu Zhao, Wei-Dong Chen, Yu-Hua Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production |
title | Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production |
title_full | Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production |
title_fullStr | Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production |
title_full_unstemmed | Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production |
title_short | Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production |
title_sort | atg7 regulates brain angiogenesis via nf-κb-dependent il-6 production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454881/ https://www.ncbi.nlm.nih.gov/pubmed/28467355 http://dx.doi.org/10.3390/ijms18050968 |
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