Cargando…

A Novel Porcine Graft for Regeneration of Bone Defects

Bone regeneration procedures require alternative graft biomaterials to those for autogenous bone. Therefore, we developed a novel porcine graft using particle sizes of 250–500 μm and 500–1000 μm in rabbit calvarial bone defects and compared the graft properties with those of commercial hydroxyapatit...

Descripción completa

Detalles Bibliográficos
Autores principales: Salamanca, Eisner, Lee, Wei-Fang, Lin, Chin-Yi, Huang, Haw-Ming, Lin, Che-Tong, Feng, Sheng-Wei, Chang, Wei-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5455581/
http://dx.doi.org/10.3390/ma8052523
_version_ 1783241072376283136
author Salamanca, Eisner
Lee, Wei-Fang
Lin, Chin-Yi
Huang, Haw-Ming
Lin, Che-Tong
Feng, Sheng-Wei
Chang, Wei-Jen
author_facet Salamanca, Eisner
Lee, Wei-Fang
Lin, Chin-Yi
Huang, Haw-Ming
Lin, Che-Tong
Feng, Sheng-Wei
Chang, Wei-Jen
author_sort Salamanca, Eisner
collection PubMed
description Bone regeneration procedures require alternative graft biomaterials to those for autogenous bone. Therefore, we developed a novel porcine graft using particle sizes of 250–500 μm and 500–1000 μm in rabbit calvarial bone defects and compared the graft properties with those of commercial hydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP) over eight weeks. Surgery was performed in 20 adult male New Zealand white rabbits. During a standardized surgical procedure, four calvarial critical-size defects of 5 mm diameter and 3 mm depth were prepared. The defects were filled with HA/β-TCP, 250–500 μm or 500–1000 μm porcine graft, and control defects were not filled. The animals were grouped for sacrifice at 1, 2, 4, and 8 weeks post-surgery. Subsequently, sample blocks were prepared for micro-computed tomography (micro-CT) scanning and histological sectioning. Similar bone formations were observed in all three treatment groups, although the 250–500 μm porcine graft performed slightly better. Rabbit calvarial bone tissue positively responded to porcine grafts and commercial HA/β-TCP, structural analyses showed similar crystallinity and porosity of the porcine and HA/β-TCP grafts, which facilitated bone formation through osteoconduction. These porcine grafts can be considered as graft substitutes, although further development is required for clinical applications.
format Online
Article
Text
id pubmed-5455581
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-54555812017-07-28 A Novel Porcine Graft for Regeneration of Bone Defects Salamanca, Eisner Lee, Wei-Fang Lin, Chin-Yi Huang, Haw-Ming Lin, Che-Tong Feng, Sheng-Wei Chang, Wei-Jen Materials (Basel) Article Bone regeneration procedures require alternative graft biomaterials to those for autogenous bone. Therefore, we developed a novel porcine graft using particle sizes of 250–500 μm and 500–1000 μm in rabbit calvarial bone defects and compared the graft properties with those of commercial hydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP) over eight weeks. Surgery was performed in 20 adult male New Zealand white rabbits. During a standardized surgical procedure, four calvarial critical-size defects of 5 mm diameter and 3 mm depth were prepared. The defects were filled with HA/β-TCP, 250–500 μm or 500–1000 μm porcine graft, and control defects were not filled. The animals were grouped for sacrifice at 1, 2, 4, and 8 weeks post-surgery. Subsequently, sample blocks were prepared for micro-computed tomography (micro-CT) scanning and histological sectioning. Similar bone formations were observed in all three treatment groups, although the 250–500 μm porcine graft performed slightly better. Rabbit calvarial bone tissue positively responded to porcine grafts and commercial HA/β-TCP, structural analyses showed similar crystallinity and porosity of the porcine and HA/β-TCP grafts, which facilitated bone formation through osteoconduction. These porcine grafts can be considered as graft substitutes, although further development is required for clinical applications. MDPI 2015-05-12 /pmc/articles/PMC5455581/ http://dx.doi.org/10.3390/ma8052523 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salamanca, Eisner
Lee, Wei-Fang
Lin, Chin-Yi
Huang, Haw-Ming
Lin, Che-Tong
Feng, Sheng-Wei
Chang, Wei-Jen
A Novel Porcine Graft for Regeneration of Bone Defects
title A Novel Porcine Graft for Regeneration of Bone Defects
title_full A Novel Porcine Graft for Regeneration of Bone Defects
title_fullStr A Novel Porcine Graft for Regeneration of Bone Defects
title_full_unstemmed A Novel Porcine Graft for Regeneration of Bone Defects
title_short A Novel Porcine Graft for Regeneration of Bone Defects
title_sort novel porcine graft for regeneration of bone defects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5455581/
http://dx.doi.org/10.3390/ma8052523
work_keys_str_mv AT salamancaeisner anovelporcinegraftforregenerationofbonedefects
AT leeweifang anovelporcinegraftforregenerationofbonedefects
AT linchinyi anovelporcinegraftforregenerationofbonedefects
AT huanghawming anovelporcinegraftforregenerationofbonedefects
AT linchetong anovelporcinegraftforregenerationofbonedefects
AT fengshengwei anovelporcinegraftforregenerationofbonedefects
AT changweijen anovelporcinegraftforregenerationofbonedefects
AT salamancaeisner novelporcinegraftforregenerationofbonedefects
AT leeweifang novelporcinegraftforregenerationofbonedefects
AT linchinyi novelporcinegraftforregenerationofbonedefects
AT huanghawming novelporcinegraftforregenerationofbonedefects
AT linchetong novelporcinegraftforregenerationofbonedefects
AT fengshengwei novelporcinegraftforregenerationofbonedefects
AT changweijen novelporcinegraftforregenerationofbonedefects