Cargando…
A Novel Porcine Graft for Regeneration of Bone Defects
Bone regeneration procedures require alternative graft biomaterials to those for autogenous bone. Therefore, we developed a novel porcine graft using particle sizes of 250–500 μm and 500–1000 μm in rabbit calvarial bone defects and compared the graft properties with those of commercial hydroxyapatit...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5455581/ http://dx.doi.org/10.3390/ma8052523 |
_version_ | 1783241072376283136 |
---|---|
author | Salamanca, Eisner Lee, Wei-Fang Lin, Chin-Yi Huang, Haw-Ming Lin, Che-Tong Feng, Sheng-Wei Chang, Wei-Jen |
author_facet | Salamanca, Eisner Lee, Wei-Fang Lin, Chin-Yi Huang, Haw-Ming Lin, Che-Tong Feng, Sheng-Wei Chang, Wei-Jen |
author_sort | Salamanca, Eisner |
collection | PubMed |
description | Bone regeneration procedures require alternative graft biomaterials to those for autogenous bone. Therefore, we developed a novel porcine graft using particle sizes of 250–500 μm and 500–1000 μm in rabbit calvarial bone defects and compared the graft properties with those of commercial hydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP) over eight weeks. Surgery was performed in 20 adult male New Zealand white rabbits. During a standardized surgical procedure, four calvarial critical-size defects of 5 mm diameter and 3 mm depth were prepared. The defects were filled with HA/β-TCP, 250–500 μm or 500–1000 μm porcine graft, and control defects were not filled. The animals were grouped for sacrifice at 1, 2, 4, and 8 weeks post-surgery. Subsequently, sample blocks were prepared for micro-computed tomography (micro-CT) scanning and histological sectioning. Similar bone formations were observed in all three treatment groups, although the 250–500 μm porcine graft performed slightly better. Rabbit calvarial bone tissue positively responded to porcine grafts and commercial HA/β-TCP, structural analyses showed similar crystallinity and porosity of the porcine and HA/β-TCP grafts, which facilitated bone formation through osteoconduction. These porcine grafts can be considered as graft substitutes, although further development is required for clinical applications. |
format | Online Article Text |
id | pubmed-5455581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54555812017-07-28 A Novel Porcine Graft for Regeneration of Bone Defects Salamanca, Eisner Lee, Wei-Fang Lin, Chin-Yi Huang, Haw-Ming Lin, Che-Tong Feng, Sheng-Wei Chang, Wei-Jen Materials (Basel) Article Bone regeneration procedures require alternative graft biomaterials to those for autogenous bone. Therefore, we developed a novel porcine graft using particle sizes of 250–500 μm and 500–1000 μm in rabbit calvarial bone defects and compared the graft properties with those of commercial hydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP) over eight weeks. Surgery was performed in 20 adult male New Zealand white rabbits. During a standardized surgical procedure, four calvarial critical-size defects of 5 mm diameter and 3 mm depth were prepared. The defects were filled with HA/β-TCP, 250–500 μm or 500–1000 μm porcine graft, and control defects were not filled. The animals were grouped for sacrifice at 1, 2, 4, and 8 weeks post-surgery. Subsequently, sample blocks were prepared for micro-computed tomography (micro-CT) scanning and histological sectioning. Similar bone formations were observed in all three treatment groups, although the 250–500 μm porcine graft performed slightly better. Rabbit calvarial bone tissue positively responded to porcine grafts and commercial HA/β-TCP, structural analyses showed similar crystallinity and porosity of the porcine and HA/β-TCP grafts, which facilitated bone formation through osteoconduction. These porcine grafts can be considered as graft substitutes, although further development is required for clinical applications. MDPI 2015-05-12 /pmc/articles/PMC5455581/ http://dx.doi.org/10.3390/ma8052523 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Salamanca, Eisner Lee, Wei-Fang Lin, Chin-Yi Huang, Haw-Ming Lin, Che-Tong Feng, Sheng-Wei Chang, Wei-Jen A Novel Porcine Graft for Regeneration of Bone Defects |
title | A Novel Porcine Graft for Regeneration of Bone Defects |
title_full | A Novel Porcine Graft for Regeneration of Bone Defects |
title_fullStr | A Novel Porcine Graft for Regeneration of Bone Defects |
title_full_unstemmed | A Novel Porcine Graft for Regeneration of Bone Defects |
title_short | A Novel Porcine Graft for Regeneration of Bone Defects |
title_sort | novel porcine graft for regeneration of bone defects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5455581/ http://dx.doi.org/10.3390/ma8052523 |
work_keys_str_mv | AT salamancaeisner anovelporcinegraftforregenerationofbonedefects AT leeweifang anovelporcinegraftforregenerationofbonedefects AT linchinyi anovelporcinegraftforregenerationofbonedefects AT huanghawming anovelporcinegraftforregenerationofbonedefects AT linchetong anovelporcinegraftforregenerationofbonedefects AT fengshengwei anovelporcinegraftforregenerationofbonedefects AT changweijen anovelporcinegraftforregenerationofbonedefects AT salamancaeisner novelporcinegraftforregenerationofbonedefects AT leeweifang novelporcinegraftforregenerationofbonedefects AT linchinyi novelporcinegraftforregenerationofbonedefects AT huanghawming novelporcinegraftforregenerationofbonedefects AT linchetong novelporcinegraftforregenerationofbonedefects AT fengshengwei novelporcinegraftforregenerationofbonedefects AT changweijen novelporcinegraftforregenerationofbonedefects |