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Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates

Unraveling the mechanisms behind the processes of cell attachment and the enhanced proliferation that occurs as a response to the presence of calcium silicate-based materials needs to be better understood so as to expand the applications of silicate-based materials. Ions in the environment may influ...

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Autores principales: Lai, Wei-Yun, Chen, Yi-Wen, Kao, Chia-Tze, Hsu, Tuan-Ti, Huang, Tsui-Hsien, Shie, Ming-You
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5455620/
https://www.ncbi.nlm.nih.gov/pubmed/28793451
http://dx.doi.org/10.3390/ma8074491
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author Lai, Wei-Yun
Chen, Yi-Wen
Kao, Chia-Tze
Hsu, Tuan-Ti
Huang, Tsui-Hsien
Shie, Ming-You
author_facet Lai, Wei-Yun
Chen, Yi-Wen
Kao, Chia-Tze
Hsu, Tuan-Ti
Huang, Tsui-Hsien
Shie, Ming-You
author_sort Lai, Wei-Yun
collection PubMed
description Unraveling the mechanisms behind the processes of cell attachment and the enhanced proliferation that occurs as a response to the presence of calcium silicate-based materials needs to be better understood so as to expand the applications of silicate-based materials. Ions in the environment may influence apatite precipitation and affect silicate ion release from silicate-based materials. Thus, the involvement of apatite precipitate in the regulation of cell behavior of human dental pulp cells (hDPCs) is also investigated in the present study, along with an investigation of the specific role of cell morphology and osteocalcin protein expression cultured on calcium silicate (CS) with different Dulbecco’s modified Eagle’s medium (DMEM). The microstructure and component of CS cement immersion in DMEM and P-free DMEM are analyzed. In addition, when hDPCs are cultured on CS with two DMEMs, we evaluate fibronectin (FN) and collagen type I (COL) secretion during the cell attachment stage. The facilitation of cell adhesion on CS has been confirmed and observed both by scanning with an electron microscope and using immunofluorescence imaging. The results indicate that CS is completely covered by an apatite layer with tiny spherical shapes on the surface in the DMEM, but not in the P-free DMEM. Compared to the P-free DMEM, the lower Ca ion in the DMEM may be attributed to the formation of the apatite on the surfaces of specimens as a result of consumption of the Ca ion from the DMEM. Similarly, the lower Si ion in the CS-soaked DMEM is attributed to the shielding effect of the apatite layer. The P-free DMEM group releases more Si ion increased COL and FN secretion, which promotes cell attachment more effectively than DMEM. This study provides new and important clues regarding the major effects of Si-induced cell behavior as well as the precipitated apatite-inhibited hDPC behavior on these materials.
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spelling pubmed-54556202017-07-28 Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates Lai, Wei-Yun Chen, Yi-Wen Kao, Chia-Tze Hsu, Tuan-Ti Huang, Tsui-Hsien Shie, Ming-You Materials (Basel) Article Unraveling the mechanisms behind the processes of cell attachment and the enhanced proliferation that occurs as a response to the presence of calcium silicate-based materials needs to be better understood so as to expand the applications of silicate-based materials. Ions in the environment may influence apatite precipitation and affect silicate ion release from silicate-based materials. Thus, the involvement of apatite precipitate in the regulation of cell behavior of human dental pulp cells (hDPCs) is also investigated in the present study, along with an investigation of the specific role of cell morphology and osteocalcin protein expression cultured on calcium silicate (CS) with different Dulbecco’s modified Eagle’s medium (DMEM). The microstructure and component of CS cement immersion in DMEM and P-free DMEM are analyzed. In addition, when hDPCs are cultured on CS with two DMEMs, we evaluate fibronectin (FN) and collagen type I (COL) secretion during the cell attachment stage. The facilitation of cell adhesion on CS has been confirmed and observed both by scanning with an electron microscope and using immunofluorescence imaging. The results indicate that CS is completely covered by an apatite layer with tiny spherical shapes on the surface in the DMEM, but not in the P-free DMEM. Compared to the P-free DMEM, the lower Ca ion in the DMEM may be attributed to the formation of the apatite on the surfaces of specimens as a result of consumption of the Ca ion from the DMEM. Similarly, the lower Si ion in the CS-soaked DMEM is attributed to the shielding effect of the apatite layer. The P-free DMEM group releases more Si ion increased COL and FN secretion, which promotes cell attachment more effectively than DMEM. This study provides new and important clues regarding the major effects of Si-induced cell behavior as well as the precipitated apatite-inhibited hDPC behavior on these materials. MDPI 2015-07-20 /pmc/articles/PMC5455620/ /pubmed/28793451 http://dx.doi.org/10.3390/ma8074491 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lai, Wei-Yun
Chen, Yi-Wen
Kao, Chia-Tze
Hsu, Tuan-Ti
Huang, Tsui-Hsien
Shie, Ming-You
Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates
title Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates
title_full Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates
title_fullStr Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates
title_full_unstemmed Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates
title_short Human Dental Pulp Cells Responses to Apatite Precipitation from Dicalcium Silicates
title_sort human dental pulp cells responses to apatite precipitation from dicalcium silicates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5455620/
https://www.ncbi.nlm.nih.gov/pubmed/28793451
http://dx.doi.org/10.3390/ma8074491
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