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Development of a method for screening short-lived proteins using green fluorescent protein
We have developed a screening technology for the identification of short-lived proteins. A green fluorescent protein (GFP)-fusion cDNA library was generated for monitoring degradation kinetics. Cells expressing a subset of the GFP-cDNA expression library were screened to recover those in which the f...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC545601/ https://www.ncbi.nlm.nih.gov/pubmed/15461799 http://dx.doi.org/10.1186/gb-2004-5-10-r81 |