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Development of a method for screening short-lived proteins using green fluorescent protein

We have developed a screening technology for the identification of short-lived proteins. A green fluorescent protein (GFP)-fusion cDNA library was generated for monitoring degradation kinetics. Cells expressing a subset of the GFP-cDNA expression library were screened to recover those in which the f...

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Detalles Bibliográficos
Autores principales: Jiang, Xin, Coffino, Philip, Li, Xianqiang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC545601/
https://www.ncbi.nlm.nih.gov/pubmed/15461799
http://dx.doi.org/10.1186/gb-2004-5-10-r81