A genome annotation-driven approach to cloning the human ORFeome

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones repres...

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Detalles Bibliográficos
Autores principales: Collins, John E, Wright, Charmain L, Edwards, Carol A, Davis, Matthew P, Grinham, James A, Cole, Charlotte G, Goward, Melanie E, Aguado, Begoña, Mallya, Meera, Mokrab, Younes, Huckle, Elizabeth J, Beare, David M, Dunham, Ian
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC545604/
https://www.ncbi.nlm.nih.gov/pubmed/15461802
http://dx.doi.org/10.1186/gb-2004-5-10-r84
Descripción
Sumario:We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.

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